Yue Wenhao, Cai Kangfeng, Xia Xue, Liu Lei, Wang Junmei
Key Laboratory of Digital Dry Land Crops of Zhejiang Province, Zhejiang Academy of Agricultural Sciences, Hangzhou, China.
National Barley Improvement Center, Hangzhou, China.
Front Plant Sci. 2023 Feb 13;14:1137434. doi: 10.3389/fpls.2023.1137434. eCollection 2023.
SWEET (Sugars Will Eventually be Exported Transporter) proteins, an essential class of sugar transporters, are involved in vital biological processes of plant growth and development. To date, systematical analysis of family in barley () has not been reported. In this study, we genome-wide identified 23 genes in barley, which were further clustered into four clades by phylogenetic tree. The members belonging to the same clade showed relatively similar gene structures and conserved protein motifs. Synteny analysis confirmed the tandem and segmental duplications among genes during evolution. Expression profile analysis demonstrated that the patterns of genes varied and the gene neofunctionalization occurred after duplications. Yeast complementary assay and subcellular localization in tobacco leaves suggested that and , highly expressed in seed aleurone and scutellum during germination, respectively, functioned as plasma membrane hexose sugar transporters. Furthermore, genetic variation detection indicated that was under artificial selection pressure during barley domestication and improvement. The obtained results facilitate our comprehensive understanding and further functional investigations of barley gene family, and also provide a potential candidate gene for domestication breeding of barley.
SWEET（糖最终输出转运蛋白）蛋白是一类重要的糖转运蛋白，参与植物生长发育的重要生物学过程。迄今为止，尚未见对大麦（）中该家族的系统分析报道。在本研究中，我们在全基因组范围内鉴定出23个大麦基因，通过系统发育树将它们进一步聚类为四个进化枝。属于同一进化枝的成员显示出相对相似的基因结构和保守的蛋白质基序。共线性分析证实了进化过程中基因间的串联重复和片段重复。表达谱分析表明，基因的模式各不相同，并且在重复后发生了基因新功能化。酵母互补试验和烟草叶片亚细胞定位表明，和分别在种子糊粉层和萌发时的盾片中高表达，作为质膜己糖转运蛋白发挥作用。此外，遗传变异检测表明，在大麦驯化和改良过程中受到人工选择压力。所获结果有助于我们全面了解大麦基因家族并进一步开展功能研究，也为大麦驯化育种提供了一个潜在的候选基因。
