College of Horticulture, Shanxi Agricultural University, Taigu, 030801, Jinzhong, China.
Datong Daylily Industrial Development Research Institute, Datong, 037000, China.
BMC Plant Biol. 2024 Jul 11;24(1):661. doi: 10.1186/s12870-024-05376-y.
Sugars will be eventually effluxed transporters (SWEETs) have been confirmed to play diverse physiological roles in plant growth, development and stress response. However, the characteristics and functions of the SWEET genes in Hemerocallis citrina remain unclear and poorly elucidated. In this study, the whole genome of Hemerocallis citrina was utilized to conduct bioinformatics analysis and a total of 19 HcSWEET genes were successfully identified. Analysis of the physicochemical properties indicated dominant differences among these HcSWEETs. A phylogenetic analysis revealed that HcSWEET proteins can be divided into 4 clades ranging from Clade I to IV, where proteins within the same clade exhibited shared conserved motifs and gene structures. Five to six exons were contained in the majority of HcSWEET genes, which were unevenly distributed across 11 chromosomes. The gene duplication analysis showed the presence of 4 gene pairs. Comparative syntenic maps revealed that the HcSWEET gene family might present more closed homology in monocotyledons than dicotyledons. Cis-acting element analysis of HcSWEET genes indicated key responsiveness to various hormones, light, and stresses. Additionally, transcriptome sequencing analysis suggested that most HcSWEET genes had a relatively higher expression in roots, and HcSWEET4a was significantly up-regulated under salt stress. Overexpression further verified the possibility that HcSWEET4a was involved in response to salt stress, which provides novel insights and facilitates in-depth studies of the functional analysis of HcSWEETs in resistance to abiotic stress.
糖转运蛋白（SWEETs）已被证实在植物生长、发育和应激反应中发挥多种生理作用。然而，萱草属植物 SWEET 基因的特征和功能仍不清楚。本研究利用萱草属植物的全基因组进行生物信息学分析，成功鉴定出 19 个 HcSWEET 基因。理化性质分析表明，这些 HcSWEET 之间存在显著差异。系统发育分析表明，HcSWEET 蛋白可分为 4 个分支，从分支 I 到分支 IV，同一分支内的蛋白具有共享的保守基序和基因结构。大多数 HcSWEET 基因包含 5 到 6 个外显子，不均匀分布在 11 条染色体上。基因复制分析表明存在 4 个基因对。比较共线性图谱表明，HcSWEET 基因家族在单子叶植物中比在双子叶植物中可能具有更高的同源性。HcSWEET 基因的顺式作用元件分析表明，它们对各种激素、光照和胁迫具有关键的响应性。此外，转录组测序分析表明，大多数 HcSWEET 基因在根中具有相对较高的表达水平，并且 HcSWEET4a 在盐胁迫下显著上调。过表达进一步验证了 HcSWEET4a 参与盐胁迫响应的可能性，为深入研究 HcSWEET 在非生物胁迫抗性中的功能分析提供了新的见解。
