Pechenova T N, Volodina T T, Nartikov A V, Popova N N, Nosal' N A
Ukr Biokhim Zh (1978). 1987 Sep-Oct;59(5):71-4.
Type I collagen in skin of rats who were given redundant amounts of leucin, valine, methionine or phenyl alanine differed essentially from the normal level in the amino acidic composition. Considerable shifts in the value of pI of the proteins under study as compared with the normal level are found during isofocusing. When studying subunits of collagen preparations by the reverse-phase chromatography certain differences typical of each studied state are revealed in the elution profiles in normalcy and under amino acid loadings.
给予过量亮氨酸、缬氨酸、蛋氨酸或苯丙氨酸的大鼠皮肤中的I型胶原蛋白，其氨基酸组成与正常水平有本质差异。在等聚焦过程中，发现所研究蛋白质的pI值与正常水平相比有相当大的变化。当通过反相色谱法研究胶原蛋白制剂的亚基时，在正常状态和氨基酸负荷状态下的洗脱图谱中揭示了每种研究状态所特有的某些差异。
