Pechenova T N, Gulyĭ M F, Volodina T T, Solodova E V, Popova N N
Ukr Biokhim Zh (1978). 1983 Jan-Feb;55(1):13-7.
Crystalline preparations of acid-soluble collagen of rat skin in norm and with tryptophan load against a background of the protein-free diet were fractionated by differential salting out using NaCl in concentrations corresponding to precipitation zones of collagen 1,3 and 4. Amino acid composition, electrophoretic mobility in polyacrylamide gel, profiles of elution from CM-cellulose, content of the carbohydrate component and--S--S-bonds were studied in proteins of the mentioned fractions and in the precipitate insoluble after the pepsin action. Essential differences as compared to the normal level in the amino acid composition and elution profiles were detected in the fraction corresponding to collagen I. Quantitative changes in the carbohydrate component and--S--S-bonds occur due to the fraction inaccessible to the persin action.
对正常大鼠皮肤以及在无蛋白饮食背景下给予色氨酸负荷的大鼠皮肤中酸溶性胶原蛋白的晶体制剂，使用对应于胶原蛋白1、3和4沉淀区浓度的氯化钠通过分级盐析进行分离。研究了上述级分的蛋白质以及胃蛋白酶作用后不溶性沉淀物中氨基酸组成、在聚丙烯酰胺凝胶中的电泳迁移率、从CM - 纤维素上的洗脱曲线、碳水化合物成分含量和二硫键情况。在对应于胶原蛋白I的级分中检测到与正常水平相比在氨基酸组成和洗脱曲线上存在本质差异。碳水化合物成分和二硫键的定量变化是由于胃蛋白酶作用不可及的级分导致的。
