Zinger Avner, Doron-Faigenboim Adi, Gelbart Dana, Levin Ilan, Lapidot Moshe
Department of Vegetable and Field Crop Research, Institute of Plant Sciences, Agricultural Research Organization - Volcani Institute, Rishon LeZion, Israel.
The Robert H. Smith Faculty of Agriculture, Food and Environment, The Hebrew University of Jerusalem, Rehovot, Israel.
PLoS Genet. 2025 May 23;21(5):e1011725. doi: 10.1371/journal.pgen.1011725. eCollection 2025 May.
Tomato brown rugose fruit virus (ToBRFV) is a rapidly spreading pathogen that poses a significant threat to tomato production worldwide. We previously identified a locus on tomato chromosome 11 controlling tolerance to the virus. We further established that combining this locus with one that maps to the Tm-1 region on chromosome 2 confers resistance to the virus. Here we sought to determine whether, and how, the Tm-1 gene itself is involved in ToBRFV resistance. Overexpression of Tm-1 in a tolerant genotype significantly reduced viral accumulation, conferring resistance to ToBRFV. On the other hand, overexpression of Tm-1 in a susceptible genotype only delayed symptom appearance. Moreover, effective RNAi-silencing of Tm-1 in the resistant genotype yielded susceptible plants. These findings show that the Tm-1 gene interacts genetically with the locus controlling tolerance on chromosome 11 and that this interaction is critical for achieving effective resistance to ToBRFV. In addition, the symptomatic plants obtained following silencing of Tm-1 in the resistant genotype indicate that tolerance is also dependent on normal expression levels of the recessive tm-1 allele.
番茄褐色皱纹果病毒（ToBRFV）是一种迅速传播的病原体，对全球番茄生产构成重大威胁。我们之前在番茄11号染色体上鉴定出一个控制对该病毒耐受性的位点。我们进一步证实，将这个位点与位于2号染色体上Tm-1区域的一个位点相结合可赋予对该病毒的抗性。在此，我们试图确定Tm-1基因本身是否以及如何参与对ToBRFV的抗性。在一个耐受性基因型中过表达Tm-1可显著降低病毒积累，赋予对ToBRFV的抗性。另一方面，在一个易感基因型中过表达Tm-1仅延迟了症状出现。此外，在抗性基因型中对Tm-1进行有效的RNA干扰沉默产生了易感植株。这些发现表明，Tm-1基因与11号染色体上控制耐受性的位点发生遗传相互作用，并且这种相互作用对于实现对ToBRFV的有效抗性至关重要。此外，在抗性基因型中沉默Tm-1后获得的有症状植株表明，耐受性也依赖于隐性tm-1等位基因的正常表达水平。
