Bharati Manisha, Rana Lokesh, Gupta Reema, Sharma Anjali, Jha Pradip K, Tomar Monika
Department of Physics and Astrophysics, University of Delhi, Delhi, 110007, India.
Department of Physics, Zakir Husain Delhi College, University of Delhi, Delhi, 110002, India.
Anal Chim Acta. 2023 Apr 8;1249:340929. doi: 10.1016/j.aca.2023.340929. Epub 2023 Feb 8.
A novel technique based on inverted Lamb wave MEMS resonator has been exploited for the realization of a DNA biosensor. Zinc oxide based Lamb wave MEMS resonator in the inverted configuration of ZnO/SiO/Si/ZnO is fabricated for label free and efficient detection of Neisseria meningitidis, responsible for bacterial meningitis. Meningitis remains a devastating endemic in sub-Saharan Africa. Its early detection can prevent the spread and its lethal complications. The developed biosensor shows a very high sensitivity of 310 Hz(ngμl) and very low detection limit of 82 pgμl for symmetric mode of the Lamb wave device while the antisymmetric mode shows a sensitivity of 202 Hz(ngμl) and the limit of detection of 84 pgμl. This very high sensitivity and very low detection limit of the Lamb wave resonator can be attributed to very high mass loading effect on the membranous structure of Lamb wave device, unlike the bulk substrate based devices. The indigenously developed MEMS based inverted Lamb wave biosensor shows high selectivity, long shelf life and good reproducibility. The ease of operation, low processing time and possibility of wireless integration of the of the Lamb wave DNA sensor paves a path towards the promising application in the field of meningitidis detection. The use of fabricated biosensor can be extended to other viral and bacterial detection applications as well.
一种基于倒置兰姆波微机电系统（MEMS）谐振器的新技术已被用于实现一种DNA生物传感器。制备了基于氧化锌的处于ZnO/SiO/Si/ZnO倒置结构的兰姆波MEMS谐振器，用于无标记且高效地检测引起细菌性脑膜炎的脑膜炎奈瑟菌。脑膜炎在撒哈拉以南非洲地区仍然是一种极具破坏性的地方病。其早期检测可以防止传播及其致命并发症。所开发的生物传感器对于兰姆波器件的对称模式显示出310 Hz/(ng/μl)的非常高的灵敏度和82 pg/μl的非常低的检测限，而反对称模式显示出202 Hz/(ng/μl)的灵敏度和84 pg/μl的检测限。与基于体基板的器件不同，兰姆波谐振器这种非常高的灵敏度和非常低的检测限可归因于对兰姆波器件膜状结构的非常高的质量负载效应。本土开发的基于MEMS的倒置兰姆波生物传感器具有高选择性、长保质期和良好的重现性。兰姆波DNA传感器操作简便、处理时间短且具有无线集成的可能性，为脑膜炎检测领域的有前景应用铺平了道路。所制备的生物传感器的用途也可以扩展到其他病毒和细菌检测应用。
