Tian Juanjuan, Zhao Xiaogan, Tang Chao, Wang Xiaomeng, Zhang Xueliang, Xiao Luyao, Li Wei
College of Food Science and Technology, Nanjing Agricultural University, Nanjing, Jiangsu 210095, PR China; College of Tea and Food Science Technology, Jiangsu Vocational College of Agriculture and Forestry, Jurong, Jiangsu 212400, PR China.
College of Food Science and Technology, Nanjing Agricultural University, Nanjing, Jiangsu 210095, PR China.
Food Res Int. 2023 Mar;165:112477. doi: 10.1016/j.foodres.2023.112477. Epub 2023 Jan 11.
The exopolysaccharide (EPS) form Paecilomyces cicadae TJJ1213 possessed immunomodulatory activity in vitro, but whether it could regulate the immune system and intestinal microbiota in vivo remained unknown. In this study, the cyclophosphamide (CTX)-induced immunosuppressive mouse model was established to explore the immunomodulatory activity of EPS. Results showed that EPS could increase the immune organ indices, promote the secretion of serum immunoglobulins and up-regulate the expression of cytokines. Additionally, EPS could repair CTX-induced intestinal injury by increasing the expression of tight junction proteins and promoting the production of short-chain fatty acids. Moreover, EPS could remarkably enhance immunity through TLR4/MyD88/NF-κB and mitogen-activated protein kinase (MAPK) signaling pathways. Furthermore, EPS regulated intestinal microbiota by increasing the abundance of beneficial bacteria (Muribaculaceae, Lachnospiraceae NK4A136, Bacteroides, Odoribacter) and reducing the level of harmful bacteria (Alistipes, Helicobacter). In conclusion, our study suggested that EPS had the abilities to enhance immunity, restore intestinal mucosal injury and modulate intestinal microbiota, and may serve as a potential prebiotic to maintain health in the future.
蝉拟青霉TJJ1213产生的胞外多糖（EPS）在体外具有免疫调节活性，但它在体内是否能调节免疫系统和肠道微生物群仍不清楚。在本研究中，建立了环磷酰胺（CTX）诱导的免疫抑制小鼠模型，以探索EPS的免疫调节活性。结果表明，EPS可增加免疫器官指数，促进血清免疫球蛋白的分泌，并上调细胞因子的表达。此外，EPS可通过增加紧密连接蛋白的表达和促进短链脂肪酸的产生来修复CTX诱导的肠道损伤。此外，EPS可通过TLR4/MyD88/NF-κB和丝裂原活化蛋白激酶（MAPK）信号通路显著增强免疫力。此外，EPS通过增加有益菌（Muribaculaceae、Lachnospiraceae NK4A136、拟杆菌属、Odoribacter）的丰度和降低有害菌（Alistipes、幽门螺杆菌）的水平来调节肠道微生物群。总之，我们的研究表明，EPS具有增强免疫力、恢复肠道黏膜损伤和调节肠道微生物群的能力，未来可能作为一种潜在的益生元来维持健康。
