Song Ju Hye, Lim Kyung Min, Yoo Sang Hyuk, Kim Gun-Dong, Shin Hee Soon, Park SungJun, Lim Mi Young, Lee So-Young
Division of Food Functionality Research, Korea Food Research Institute, 245, Nongsaengmyeong-Ro, Iseo-Myeon, Wanju-Gun, 55365, Jeollabuk-Do, Republic of Korea.
Department of Food Biotechnology, Korea, University of Science and Technology, Daejeon, Republic of Korea.
Probiotics Antimicrob Proteins. 2025 Jan 30. doi: 10.1007/s12602-025-10463-z.
This study evaluated the immune-enhancing efficacy of Limosilactobacillus fermentum KBL375 isolated from the feces of healthy Koreans. KBL375-treated splenocytes showed enhancement of cytotoxicity against YAC-1 cells, the target of natural killer (NK) cells, with an increase in CD335, granzyme B, perforin, and interferon-gamma (IFN-γ). Oral administration of KBL375 in mice with cyclophosphamide (CP)-induced immunosuppression improved body weight and immune functions, including immune organ indices, lymphocyte proliferations, and immunoglobulin (Ig) A levels. Notably, KBL375 increased NK cell cytotoxicity and proportion in immunosuppressed mice. Perforin/IFN-γ expression levels, which indicated NK cell activation, were also increased in KBL375-treated mice. Furthermore, KBL375 led to an increase in beneficial microbes, such as Bifidobacterium, in the gut microbiome of immunosuppressed mice, fostering a favorable intestinal microbial environment. These comprehensive results suggest that KBL375 exhibits potent immune regulatory functions and positively influences the gut microbiota, implying its potential as a probiotic agent for immune enhancement.
本研究评估了从健康韩国人粪便中分离出的发酵乳杆菌KBL375的免疫增强功效。经KBL375处理的脾细胞对自然杀伤（NK）细胞的靶细胞YAC-1细胞的细胞毒性增强，同时CD335、颗粒酶B、穿孔素和干扰素-γ（IFN-γ）增加。对环磷酰胺（CP）诱导免疫抑制的小鼠口服KBL375可改善体重和免疫功能，包括免疫器官指数、淋巴细胞增殖和免疫球蛋白（Ig）A水平。值得注意的是，KBL375增加了免疫抑制小鼠的NK细胞细胞毒性和比例。在经KBL375处理的小鼠中，表明NK细胞活化的穿孔素/IFN-γ表达水平也有所增加。此外，KBL375导致免疫抑制小鼠肠道微生物群中有益微生物（如双歧杆菌）增加，促进了有利的肠道微生物环境。这些综合结果表明，KBL375具有强大的免疫调节功能，并对肠道微生物群产生积极影响，这意味着它作为一种免疫增强益生菌剂具有潜力。
