Yin Honggang, Huang Junhao, Guo Xiaozheng, Xia Jingbo, Hu Min
Guangdong Provincial Key Laboratory of Sports and Health Promotion, Guangzhou Sport University, Guangzhou 510500, China.
School of Exercise and Health, Shanghai University of Sport, Shanghai 200438, China.
FEMS Microbiol Lett. 2023 Jan 17;370. doi: 10.1093/femsle/fnad016.
Our previous study revealed moderate-intensity exercise improved endothelial function associated with decreasing Romboutsia in rats on a high-fat diet. However, whether Romboutsia influences endothelial function remains unclear. The aim of this study was to determine the effects of Romboutsia lituseburensis JCM1404 on the vascular endothelium of rats under standard diet (SD) or high-fat diet (HFD). Romboutsia lituseburensis JCM1404 had a better improvement effect on endothelial function under HFD groups, while no significant effect on small-intestinal and blood vessel morphology. HFD significantly decreased villus height of small intestine and increased outer diameter and media thickness of the vascular tissue. After the treatments by R. lituseburensis JCM1404, the expression of claudin5 was increased in the HFD groups. Romboutsia lituseburensis JCM1404 was found to increase alpha diversity in the SD groups, with an increase in beta diversity in the HFD groups. The relative abundance of Romboutsia and Clostridium_sensu_stricto_1 was decreased significantly in both diet groups after R. lituseburensis JCM1404 intervention. The functions of human diseases and endocrine and metabolic diseases significantly downregulated in the HFD groups by Tax4Fun analysis. Furthermore, we found Romboutsia was significantly associated with bile acids, triglycerides, amino acids and derivatives and organic acids and derivatives in the SD groups, while Romboutsia was significantly associated with triglycerides and free fatty acid in the HFD groups. Romboutsia lituseburensis JCM1404 significantly upregulated several metabolism-related pathways by KEGG analysis in the HFD groups, including glycerolipid metabolism, cholesterol metabolism, regulation of lipolysis in adipocytes, insulin resistance, fat digestion and absorption, thermogenesis. Overall, R. lituseburensis JCM1404 supplementation ameliorated endothelial function via gut microbiota modulation and lipid metabolisms alterations in obese rats.
我们之前的研究表明，中等强度运动可改善高脂饮食大鼠的内皮功能，这与减少罗氏菌属有关。然而，罗氏菌属是否影响内皮功能仍不清楚。本研究的目的是确定里氏罗氏菌JCM1404对标准饮食（SD）或高脂饮食（HFD）大鼠血管内皮的影响。里氏罗氏菌JCM1404对高脂饮食组的内皮功能有更好的改善作用，而对小肠和血管形态无显著影响。高脂饮食显著降低了小肠绒毛高度，增加了血管组织的外径和中膜厚度。经里氏罗氏菌JCM1404处理后，高脂饮食组中claudin5的表达增加。发现里氏罗氏菌JCM1404增加了标准饮食组的α多样性，高脂饮食组的β多样性增加。里氏罗氏菌JCM1404干预后，两个饮食组中罗氏菌属和严格梭菌属_1的相对丰度均显著降低。通过Tax4Fun分析，高脂饮食组中人类疾病以及内分泌和代谢疾病的功能显著下调。此外，我们发现标准饮食组中罗氏菌属与胆汁酸、甘油三酯、氨基酸及其衍生物以及有机酸及其衍生物显著相关，而高脂饮食组中罗氏菌属与甘油三酯和游离脂肪酸显著相关。通过KEGG分析，里氏罗氏菌JCM1404在高脂饮食组中显著上调了几个与代谢相关的途径，包括甘油olipid代谢、胆固醇代谢、脂肪细胞中脂解的调节、胰岛素抵抗、脂肪消化和吸收、产热。总体而言，补充里氏罗氏菌JCM1404可通过调节肠道微生物群和改变肥胖大鼠的脂质代谢来改善内皮功能。
