Liu Zhi-Hua, Wang Jin-Lan, Zhao Ying-Nan, Li Jun, Shi Zhi-Chun, Wang Dan, Zhao Ming, Zhang Shu-Jun
College of Chemistry and Chemical Engineering, Qiqihar University Qiqihar 161006, China.
College of Chemistry and Chemical Engineering, Qiqihar University Qiqihar 161006, China Heilongjiang Industrial Hemp Processing Technology Innovation Center Qiqihar 161006, China Technology Innovation Center of Industrial Hemp for State Market Regulation Qiqihar 161006, China.
Zhongguo Zhong Yao Za Zhi. 2023 Feb;48(4):985-992. doi: 10.19540/j.cnki.cjcmm.20220406.201.
The present study investigated the chemical constituents in the aerial part of Cannabis sativa. The chemical constituents were isolated and purified by silica gel column chromatography and HPLC and identified according to their spectral data and physicochemical properties. Thirteen compounds were isolated from the acetic ether extract of C. sativa and identified as 3',5',4″,2-tetrahydroxy-4'-methoxy-3-methyl-3″-butenyl p-disubstituted benzene ethane(1), 16R-hydroxyoctadeca-9Z,12Z,14E-trienoic acid methyl ester(2),(1'R,2'R)-2'-(2-hydroxypropan-2-yl)-5'-methyl-4-pentyl-1',2',3',4'-tetrahydro-(1,1'-biphenyl)-2,6-diol(3), β-sitosteryl-3-O-β-D-glucopyranosyl-6'-O-palmitate(4), 9S,12S,13S-trihydroxy-10-octadecenoate methyl ester(5), benzyloxy-1-O-β-D-glucopyranoside(6), phenylethyl-O-β-D-glucopyranoside(7), 3Z-enol glucoside(8), α-cannabispiranol-4'-O-β-D-glucopyranose(9), 9S,12S,13S-trihydroxyoctadeca-10E,15Z-dienoic acid(10), uracil(11), o-hydroxybenzoic acid(12), and 2'-O-methyladenosine(13). Compound 1 is a new compound, compound 3 is a new natural product, and compounds 2, 4-8, 10, and 13 were isolated from Cannabis plant for the first time.
本研究对大麻地上部分的化学成分进行了研究。通过硅胶柱色谱和高效液相色谱对化学成分进行分离和纯化，并根据其光谱数据和理化性质进行鉴定。从大麻的乙酸乙酯提取物中分离出13种化合物，分别鉴定为3',5',4″,2-四羟基-4'-甲氧基-3-甲基-3″-丁烯基对二取代苯乙烷（1）、16R-羟基十八碳-9Z,12Z,14E-三烯酸甲酯（2）、(1'R,2'R)-2'-(2-羟基丙烷-2-基)-5'-甲基-4-戊基-1',2',3',4'-四氢-(1,1'-联苯)-2,6-二醇（3）、β-谷甾醇-3-O-β-D-吡喃葡萄糖基-6'-O-棕榈酸酯（4）、9S,12S,13S-三羟基-10-十八碳烯酸甲酯（5）、苄氧基-1-O-β-D-吡喃葡萄糖苷（6）、苯乙基-O-β-D-吡喃葡萄糖苷（7）、3Z-烯醇葡萄糖苷（8）、α-大麻螺烷醇-4'-O-β-D-吡喃葡萄糖（9）、9S,12S,13S-三羟基十八碳-10E,15Z-二烯酸（10）、尿嘧啶（11）、邻羟基苯甲酸（12）和2'-O-甲基腺苷（13）。化合物1为新化合物；化合物3为新天然产物；化合物2、4-8、10和13首次从大麻植物中分离得到。
