Wang Cai, Xin Dongyuan, Yue Qianwen, Wan Huiyu, Li Qian, Wang Ying, Wu Jingguo
The Second Affiliated Hospital, Shandong First Medical University & Shandong Academy of Medical Sciences, Taian, Shandong, China.
Binhai County People's Hospital, Yancheng, Jiangsu, China.
Front Bioeng Biotechnol. 2023 Feb 16;11:1128934. doi: 10.3389/fbioe.2023.1128934. eCollection 2023.
Interleukin-6 (IL-6) is a multifunctional polypeptide cytokine composed of two glycoprotein chains, which plays an important role in many cellular reactions, pathological processes, diagnosis and treatment of diseases and so on. The detection of IL-6 plays a promising role in the cognition of clinical diseases. 4-mercaptobenzoic acid (4-MBA) was immobilized on the gold nanoparticles modified platinum carbon (PC) electrode with the linker IL-6 antibody, and finally formed an electrochemical sensor that specifically recognized IL-6. Through the highly specific antigen-antibody reaction, the IL-6 concentration of the samples to be detected. The performance of the sensor was studied by cyclic voltammetry (CV) and differential pulse voltammetry (DPV). The experimental results showed that the linear detection range of the sensor for IL-6 was 100 pg/mL-700 pg/mL and the detection limit was 3 pg/mL. In addition, the sensor had the advantages of high specificity, high sensitivity, high stability and reproducibility under the interference environment of bovine serum albumin (BSA), glutathione (GSH), glycine (Gly) and neuron specific enolase (NSE), which provided a prospect for specific antigen detection sensor. The prepared electrochemical sensor successfully detected the content of IL-6 in standard and biological samples, showing excellent detection performance. No significant difference was found between the detection results of the sensor and that of ELISA. The sensor showed a very broad prospect in the application and detection of clinical samples.
白细胞介素-6（IL-6）是一种由两条糖蛋白链组成的多功能多肽细胞因子，在许多细胞反应、病理过程、疾病的诊断和治疗等方面发挥着重要作用。IL-6的检测在临床疾病的认知中具有重要作用。4-巯基苯甲酸（4-MBA）通过连接子IL-6抗体固定在金纳米粒子修饰的铂碳（PC）电极上，最终形成一种特异性识别IL-6的电化学传感器。通过高度特异性的抗原-抗体反应，检测待检测样品中的IL-6浓度。采用循环伏安法（CV）和差分脉冲伏安法（DPV）研究了该传感器的性能。实验结果表明，该传感器对IL-6的线性检测范围为100 pg/mL - 700 pg/mL，检测限为3 pg/mL。此外，该传感器在牛血清白蛋白（BSA）、谷胱甘肽（GSH）、甘氨酸（Gly）和神经元特异性烯醇化酶（NSE）的干扰环境下具有高特异性、高灵敏度、高稳定性和重现性等优点，为特异性抗原检测传感器提供了广阔前景。所制备的电化学传感器成功检测了标准样品和生物样品中IL-6的含量，表现出优异的检测性能。该传感器的检测结果与酶联免疫吸附测定（ELISA）的检测结果无显著差异。该传感器在临床样品的应用和检测中显示出非常广阔的前景。
