Mady Mohamed S, Ibrahim Reham R, El-Sayed Elsayed K, El-Shazly Mohamed, Chen Lo-Yun, Lai Kuei-Hung, El Shaarawy Fatheya S, Moharram Fatma A
Faculty of Pharmacy, Pharmacognosy Department, Helwan University, Cairo, Egypt.
Faculty of Pharmacy, Pharmacology and Toxicology Department, Helwan University, Cairo, Egypt.
Front Pharmacol. 2023 Feb 17;14:1120146. doi: 10.3389/fphar.2023.1120146. eCollection 2023.
L. genus (Oak) belongs to the family Fagaceae and their galls are used commercially in leather tanning, dyeing, and ink preparation. Several species were traditionally used to manage wound healing, acute diarrhea, hemorrhoid, and inflammatory diseases. The present study aims to investigate the phenolic content of the 80% aqueous methanol extract (AME) of and leaves as well as to assess their anti-diarrheal activity. Polyphenolic content of and AME were investigated using UHPLC/MS. The antidiarrheal potential of the obtained extracts was evaluated by conducting a castor oil-induced diarrhea model. Twenty-five and twenty-six polyphenolic compounds were tentatively identified in and AME, respectively. The identified compounds are related to quercetin, kaempferol, isorhamnetin, and apigenin glycosides and their aglycones. In addition, hydrolyzable tannins, phenolic acid, phenyl propanoides derivatives, and cucurbitacin F were also identified in both species AME of (250, 500, and 1000 mg/kg) exhibited a significant prolongation in the onset of diarrhea by 17.7 %, 42.6%, and 79.7% respectively while AME of at the same doses significantly prolonged the onset of diarrhea by 38.6%, 77.3%, and 2.4 folds respectively as compared to the control. Moreover, the percentage of diarrheal inhibition of was 23.8%, 28.57%, and 42,86% respectively, and for 33.34%, 47.3%, and 57.14% respectively as compared to the control group. Both extracts significantly decreased the volume of intestinal fluid by 27%, 39.78%, and 50.1% for respectively; and by 38.71%, 51.19%, and 60% for respectively as compared to the control group. In addition, AME of exhibited a peristaltic index of 53.48, 47.18, and 42.28 with significant inhibition of gastrointestinal transit by 18.98%, 28.53%, and 35.95 % respectively; while AME of exhibited a peristaltic index of 47.71, 37, and 26.41 with significant inhibition of gastrointestinal transit by 27.72%, 43.89%, and 59.99% respectively as compared with the control group. Notably, showed a better antidiarrheal effect in comparison with and, the highest effect was observed for at 1000 mg/kg as it was nonsignificant from the loperamide standard group in all measured parameters.
栎属（橡树）属于壳斗科，其瘿瘤在皮革鞣制、染色和油墨制备中具有商业用途。传统上，有几种栎属植物被用于处理伤口愈合、急性腹泻、痔疮和炎症性疾病。本研究旨在研究[栎属植物名称1]和[栎属植物名称2]叶片80%甲醇水溶液提取物（AME）中的酚类成分，并评估其抗腹泻活性。使用超高效液相色谱/质谱法研究了[栎属植物名称1]和[栎属植物名称2] AME中的多酚含量。通过蓖麻油诱导的腹泻模型评估所得提取物的抗腹泻潜力。在[栎属植物名称1]和[栎属植物名称2] AME中分别初步鉴定出25种和26种多酚化合物。鉴定出的化合物与槲皮素、山奈酚、异鼠李素和芹菜素糖苷及其苷元有关。此外，在两种栎属植物AME中还鉴定出了可水解单宁、酚酸、苯丙烷类衍生物和葫芦素F。[栎属植物名称1]的AME（250、500和1000mg/kg）分别使腹泻发作时间显著延长17.7%、42.6%和79.7%，而相同剂量的[栎属植物名称2] AME与对照组相比，腹泻发作时间分别显著延长38.6%、77.3%和2.4倍。此外，与对照组相比，[栎属植物名称1]的腹泻抑制率分别为23.8%、28.57%和42.86%，[栎属植物名称2]的腹泻抑制率分别为33.34%、47.3%和57.14%。两种提取物均使肠液体积显著减少，[栎属植物名称1]分别减少27%、39.78%和50.1%；[栎属植物名称2]分别减少38.71%、51.19%和60%。此外，[栎属植物名称1]的AME蠕动指数分别为53.48、47.18和42.28，胃肠道转运分别显著抑制18.98%、28.53%和35.95%；而[栎属植物名称2]的AME蠕动指数分别为47.71、37和26.41，胃肠道转运分别显著抑制27.72%、43.89%和59.99%。值得注意的是，与[栎属植物名称2]相比，[栎属植物名称1]显示出更好的抗腹泻效果，在1000mg/kg时观察到最高效果，因为在所有测量参数中与洛哌丁胺标准组无显著差异。
