Malespín-Bendaña Wendy, Alpízar-Alpízar Warner, Figueroa-Protti Lucía, Reyes Ledis, Molina-Castro Silvia, Une Clas, Ramírez-Mayorga Vanessa
Institute of Health Research (INISA), University of Costa Rica, San José, Costa Rica.
Centre for Research on Microscopic Structures (CIEMic), University of Costa Rica, San José, Costa Rica.
Front Oncol. 2023 Feb 15;13:1072802. doi: 10.3389/fonc.2023.1072802. eCollection 2023.
colonizes the gastric mucosa and induces chronic inflammation.
Using a mouse model of -induced gastritis, we evaluated the mRNA and protein expression levels of proinflammatory and proangiogenic factors, as well as the histopathological changes in gastric mucosa in response to infection. Five- to six-week-old female C57BL/6N mice were challenged with SS1 strain. Animals were euthanized after 5-, 10-, 20-, 30-, 40- and 50-weeks post infection. mRNA and protein expression of Angpt1, Angpt2, VegfA, Tnf-α, bacterial colonization, inflammatory response and gastric lesions were evaluated.
A robust bacterial colonization was observed in 30 to 50 weeks-infected mice, which was accompanied by immune cell infiltration in the gastric mucosa. Compared to non-infected animals, colonized animals showed an upregulation in the expression of , and at the mRNA and protein levels. In contrast, mRNA and protein expression was downregulated in -colonized mice.
Our data show that infection induces the expression of Angpt2, and Vegf-A in murine gastric epithelium. This may contribute to the pathogenesis of -associated gastritis, however the significance of this should be further addressed.
定殖于胃黏膜并引发慢性炎症。
利用诱导性胃炎小鼠模型，我们评估了促炎和促血管生成因子的mRNA和蛋白表达水平，以及胃黏膜对感染的组织病理学变化。用SS1菌株对5至6周龄的雌性C57BL/6N小鼠进行攻击。在感染后5、10、20、30、40和50周对动物实施安乐死。评估血管生成素1（Angpt1）、血管生成素2（Angpt2）、血管内皮生长因子A（VegfA）、肿瘤坏死因子-α（Tnf-α）的mRNA和蛋白表达、细菌定殖、炎症反应及胃部病变情况。
在感染30至50周的小鼠中观察到大量细菌定殖，同时伴有胃黏膜免疫细胞浸润。与未感染动物相比，定殖动物的血管生成素2、肿瘤坏死因子-α和血管内皮生长因子A在mRNA和蛋白水平表达上调。相反，在定殖小鼠中肿瘤坏死因子-α的mRNA和蛋白表达下调。
我们的数据表明，感染诱导小鼠胃上皮细胞中血管生成素2、肿瘤坏死因子-α和血管内皮生长因子A的表达。这可能促成相关胃炎的发病机制，不过其意义仍需进一步探讨。
