Ramadan Ahmed M, Mohammed Taimyiah, Al-Ghamdi Khalid M, Alghamdi Abdullah J, Atef Ahmed
Biological Science Department, Faculty of Science, King Abdulaziz University, Jeddah, Saudi Arabia.
Princess Najla bint Saud Al-Saud Center for Excellence Research in Biotechnology, King Abdulaziz University, Jeddah, Saudi Arabia.
Saudi J Biol Sci. 2023 Mar;30(3):103600. doi: 10.1016/j.sjbs.2023.103600. Epub 2023 Feb 15.
Genomic studies not only help researcher not only to identify genomic features in organisms, but also facilitate understanding of evolutionary relationships. Species in the genus have medicinal benefits, and one of them is which is used to treat various diseases. This report investigates the nucleotides and genic features of chloroplast genome of and trying to clarify the evolutionary relationship with and family Solanaceae. We found that the total size of genome was 153.771 kb (the smallest chloroplast genome in genus Withania). A large single-copy region (91.285 kb), a small single-copy region (18.373 kb) form the genomic region, and are distinct from each other by a large inverted repeat (22.056 kb). 137 chloroplast genes are found including 4 rRNAs, 38 tRNAs and 83 protein-coding genes. The chloroplast genome as well as four closest relatives was compared for features such as structure, nucleotide composition, simple sequence repeats (SSRs) and codon bias. Compared to other species, has unique characteristics. It has the smallest chloroplast genome of any species, isoleucine is the major amino acid, and tryptophan is the minor, In addition, there are no and genes, fourth, there are only fifteen replicative genes, while in most other species there are more. Using fast minimum evolution and neighbor joining, we have reconstructed the trees to confirm the relationship with other Solanacaea species. The chloroplast genome is submitted under accession no. ON153173.
基因组研究不仅有助于研究人员识别生物体中的基因组特征，还能促进对进化关系的理解。该属中的物种具有药用价值，其中一种被用于治疗各种疾病。本报告研究了[物种名称]叶绿体基因组的核苷酸和基因特征，并试图阐明其与[其他物种名称]和茄科的进化关系。我们发现[物种名称]基因组的总大小为153.771 kb（是Withania属中最小的叶绿体基因组）。一个大单拷贝区域（91.285 kb）、一个小单拷贝区域（18.373 kb）构成了基因组区域，它们通过一个大的反向重复序列（22.056 kb）彼此区分。共发现137个叶绿体基因，包括4个rRNA、38个tRNA和83个蛋白质编码基因。对[物种名称]叶绿体基因组以及四个最亲近的亲属进行了结构、核苷酸组成、简单序列重复（SSR）和密码子偏好等特征的比较。与其他[物种名称]物种相比，[物种名称]具有独特的特征。它是所有[物种名称]物种中叶绿体基因组最小的，异亮氨酸是主要氨基酸，色氨酸是次要氨基酸，此外，没有[特定基因名称1]和[特定基因名称2]基因，第四，只有十五个复制基因，而在大多数其他物种中更多。使用快速最小进化法和邻接法，我们重建了系统发育树以确认其与其他茄科物种的关系。[物种名称]叶绿体基因组已提交，登录号为ON153173。
