表达Cas9和T7 RNA聚合酶的MNYC/BZ/62/M379的基因组序列。
Genome sequence of MNYC/BZ/62/M379 expressing Cas9 and T7 RNA polymerase.
作者信息
Beneke Tom, Dobramysl Ulrich, Catta-Preta Carolina Moura Costa, Mottram Jeremy Charles, Gluenz Eva, Wheeler Richard J
机构信息
Sir William Dunn School of Pathology, University of Oxford, Oxford, UK.
Department of Cell and Developmental Biology, Biocentre, University of Würzburg, Würzburg, 97074, Germany.
出版信息
Wellcome Open Res. 2023 Feb 23;7:294. doi: 10.12688/wellcomeopenres.18575.2. eCollection 2022.
We present the genome sequence of MNYC/BZ/62/M379 modified to express Cas9 and T7 RNA-polymerase, revealing high similarity to the reference genome (MHOM/GT2001/U1103). Through RNAseq-based annotation of coding sequences and untranslated regions, we provide primer sequences for construct and sgRNA template generation for CRISPR-assisted gene deletion and endogenous tagging.
我们展示了经修饰以表达Cas9和T7 RNA聚合酶的MNYC/BZ/62/M379的基因组序列，发现其与参考基因组（MHOM/GT2001/U1103）高度相似。通过基于RNAseq对编码序列和非翻译区进行注释，我们提供了用于构建体和sgRNA模板生成的引物序列，以用于CRISPR辅助的基因缺失和内源性标记。
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