Zacour M E, Collier B, Martin J G
Meakins-Christie Laboratories, McGill University, Montreal, Quebec, Canada.
Agents Actions. 1987 Oct;22(1-2):75-81. doi: 10.1007/BF01968820.
Apamin has been shown to antagonize the nonadrenergic, noncholinergic (NANC) inhibitory system in guinea pig taenia coli. We have examined the effects of apamin on the nonadrenergic noncholinergic inhibitory system and its putative transmitters in isolated guinea pig trachea. Electrical field stimulation (ES) of isolated trachea pretreated with atropine and propranolol evoked reproducible relaxations that were blocked by tetrodoxin, but were unaffected by apamin. Vasoactive intestinal peptide (VIP), adenosine (AD), and adenosine triphosphate (ATP) produced concentration-dependent inhibition of histamine (H)-induced contractions of isolated trachea but the inhibitory actions of these agents were not significantly affected by apamin. In contrast, apamin virtually abolished ES-evoked relaxations in guinea pig isolated taenia caeci, and reduced the inhibition of H-induced contraction by ATP from 40% to 1%. We conclude that neither the NANC inhibitory system in the guinea pig trachea nor its putative mediators VIP, AD, and ATP are antagonized by apamin, in contrast to taenia caeci.
蜂毒明肽已被证明可拮抗豚鼠结肠带中的非肾上腺素能、非胆碱能（NANC）抑制系统。我们研究了蜂毒明肽对豚鼠离体气管中非肾上腺素能非胆碱能抑制系统及其假定递质的影响。用阿托品和普萘洛尔预处理的离体气管进行电场刺激（ES）可引起可重复的舒张，这种舒张可被河豚毒素阻断，但不受蜂毒明肽影响。血管活性肠肽（VIP）、腺苷（AD）和三磷酸腺苷（ATP）对组胺（H）诱导的离体气管收缩产生浓度依赖性抑制，但这些药物的抑制作用不受蜂毒明肽的显著影响。相比之下，蜂毒明肽实际上消除了豚鼠离体盲肠带中ES诱发的舒张，并使ATP对H诱导收缩的抑制作用从40%降至1%。我们得出结论，与盲肠带不同，豚鼠气管中的NANC抑制系统及其假定介质VIP、AD和ATP均不受蜂毒明肽的拮抗。
