Krovacek K, Faris A, Eriksson L, Jansson E, Ljungberg O, Månsson I
Acta Vet Scand. 1987;28(1):47-54. doi: 10.1186/BF03548255.
Extracellular products in culture filtrates of Aeromonas salmonicida subsp. achromogenes and Vibrio anguillarum isolated from infected fish have been shown to possess skin inflammatory factor. The extracellular products from Vibrio anguillarum were cytotoxic in HeLa and CHO cells. In addition to the skin lesions, the culture filtrates of V. anguillarum caused necrotic reaction on the rabbit skin. Five of 6 strains of V. anguillarum were lethal to mice after intraperitoneal administration of 3×10 CFU. Only 1 strain of 5 A. salmonicida subsp. achromogenes produced extracellular products which elicited cytotoxic effects in the CHO cells. None of the A. salmonicida subsp. achromogenes strains were lethal to mice. The cytotoxins were inactivated when heated at 65°C for 30 min. The results indicate that the thermolabile exotoxins are non-enterotoxic since they failed to stimulate fluid accumulation in the rabbit ileal loop and did not cause elongation of the CHO cells. The rounding off of CHO cells, as well as of HeLa cells indicate that the exotoxins may play an important role in fish diseases.
从感染鱼体分离出的杀鲑气单胞菌无色亚种和鳗弧菌培养滤液中的细胞外产物已被证明具有皮肤炎症因子。鳗弧菌的细胞外产物对HeLa细胞和CHO细胞具有细胞毒性。除皮肤损伤外，鳗弧菌的培养滤液对兔皮肤会引起坏死反应。6株鳗弧菌中的5株在腹腔注射3×10 CFU后对小鼠具有致死性。5株杀鲑气单胞菌无色亚种中只有1株产生的细胞外产物在CHO细胞中引发了细胞毒性作用。杀鲑气单胞菌无色亚种的菌株对小鼠均无致死性。细胞毒素在65°C加热30分钟后失活。结果表明，这些热不稳定外毒素是非肠毒素性的，因为它们未能刺激兔回肠袢中的液体蓄积，也未导致CHO细胞伸长。CHO细胞以及HeLa细胞的变圆表明外毒素可能在鱼类疾病中起重要作用。
