Medical Research Centre, Changzhou Second People's Hospital Affiliated to Nanjing Medical University, Changzhou, 213164, People's Republic of China.
Department of Clinical Laboratory, Changzhou Second People's Hospital Affiliated to Nanjing Medical University, Changzhou, 213164, People's Republic of China.
Int J Nanomedicine. 2021 Dec 30;16:8417-8432. doi: 10.2147/IJN.S339500. eCollection 2021.
Bone tissue engineering (BTE) is a new strategy for bone defect repair, but the difficulties in the fabrication of scaffolds with personalized structures still limited their clinical applications. The rapid development in three-dimensional (3D) printing endows it capable of controlling the porous structures of scaffolds with high structural complexity and provides flexibility to meet specific needs of bone repair.
In this study, sodium alginate (SA)/gelatin (Gel) hydrogel scaffolds doped with different contents of nano-attapulgite were fabricated via 3D printing. The surface microstructure, hydrophilicity and mechanical properties were fully evaluated. Furthermore, mouse bone marrow-derived mesenchymal stem cells (BMSCs) were cultured with the composite hydrogels in vitro, and proliferation and osteoblastic differentiation were assessed. A rabbit tibia plateau defect model was used to evaluate the osteogenic potential of the composite hydrogel in vivo.
When increasing nano-ATP content, the Gel/SA/nano-ATP composite hydrogels showed better mechanical property and printability. Moreover, Gel/SA/nano-ATP composite hydrogels showed excellent bioactivity, and a significant mineralization effect was observed on the surface after being incubated in simulated body fluid (SBF) for 14 days. The Gel/SA/nano-ATP composite hydrogel also showed good biocompatibility and promoted the osteogenesis of BMSCs. Finally, histological analysis demonstrates that the Gel/SA/nano-ATP composite hydrogels could effectively enhance bone regeneration in vivo.
These properties render the Gel/SA/nano-ATP composite hydrogel scaffolds an ideal bone tissue engineering material for the repair of bone defects.
骨组织工程（BTE）是修复骨缺损的新策略，但制造具有个性化结构支架的困难仍然限制了其临床应用。三维（3D）打印的快速发展使其能够控制支架的多孔结构，具有高度复杂的结构，并提供灵活性以满足骨修复的特定需求。
在这项研究中，通过 3D 打印制备了不同纳米凹凸棒土含量掺杂的海藻酸钠（SA）/明胶（Gel）水凝胶支架。充分评估了表面微观结构、亲水性和机械性能。此外，体外培养小鼠骨髓间充质干细胞（BMSCs）与复合水凝胶，评估增殖和成骨分化。使用兔胫骨平台缺损模型评估复合水凝胶的体内成骨潜力。
随着纳米-ATP 含量的增加，Gel/SA/nano-ATP 复合水凝胶表现出更好的机械性能和可打印性。此外，Gel/SA/nano-ATP 复合水凝胶具有优异的生物活性，在模拟体液（SBF）中孵育 14 天后，表面观察到明显的矿化效果。Gel/SA/nano-ATP 复合水凝胶还表现出良好的生物相容性，促进 BMSCs 的成骨作用。最后，组织学分析表明，Gel/SA/nano-ATP 复合水凝胶可有效促进体内骨再生。
这些特性使 Gel/SA/nano-ATP 复合水凝胶支架成为修复骨缺损的理想骨组织工程材料。
