Lunawat Kuhu, Kavitha S, Rajkumar G, Dhivya V, Ravi Kumar N, Mahalaxmi S, Shaik Fayaz Ahmed
Department of Conservative Dentistry and Endodontics, SRM Institute of Science and Technology, Ramapuram, Chennai, 600 089, Tamil Nadu, India.
Department of Conservative Dentistry and Endodontics, SRM Institute of Science and Technology, Ramapuram, Chennai, 600 089, Tamil Nadu, India.
J Mech Behav Biomed Mater. 2023 Apr;140:105750. doi: 10.1016/j.jmbbm.2023.105750. Epub 2023 Mar 1.
This study aimed to evaluate the effect of incorporation of strontium based fluoro phosphate glass (SrFPG) 48PO-29CaO-14NaO-3CaF-6SrO on physico chemical and biological properties of mineral trioxide aggregate (MTA).
Optimized SrFPG glass powder were prepared using planetary ball mill and incorporated into MTA in varying proportion (1, 5, 10 wt%) to obtain SrMT1, SrMT5, SrMT10 bio-composite respectively. The bio-composites were characterized using XRD, FTIR and SEM-EDAX before and after soaking for 28 days in stimulated body fluid (SBF) solution. To assess the mechanical properties and biocompatibility of the prepared bio-composite, density, pH analysis, compressive strength and cytotoxicity evaluation using MTT assay were done before and after soaking for 28 days in SBF solution.
A nonlinear variation in compressive strength and pH values was noted. Of the bio-composites, SrMT10 was evidenced with rich apatite formation in XRD, FTIR and SEM with EDAX analysis. MTT assay showed increased cell viability in all the samples before and after in vitro studies.
本研究旨在评估掺入锶基氟磷酸盐玻璃（SrFPG）48PO-29CaO-14NaO-3CaF-6SrO对三氧化物矿物聚合体（MTA）物理化学和生物学性质的影响。
使用行星式球磨机制备优化的SrFPG玻璃粉末，并以不同比例（1、5、10重量%）掺入MTA中，分别获得SrMT1、SrMT5、SrMT10生物复合材料。在模拟体液（SBF）溶液中浸泡28天前后，使用X射线衍射（XRD）、傅里叶变换红外光谱（FTIR）和扫描电子显微镜-能谱分析（SEM-EDAX）对生物复合材料进行表征。为了评估制备的生物复合材料的力学性能和生物相容性，在SBF溶液中浸泡28天前后，进行密度、pH分析、抗压强度和使用MTT法的细胞毒性评估。
观察到抗压强度和pH值的非线性变化。在生物复合材料中，通过XRD、FTIR和带有能谱分析的SEM证实，SrMT10有丰富的磷灰石形成。MTT法显示，在体外研究前后，所有样品中的细胞活力均有所增加。
