Department of Sport Sciences, Faculty of Humanities, Semnan University, Semnan, Iran.
Associate Professor of Exercise Physiology, Department of Sport Sciences, Faculty of Humanities, Semnan University, Semnan, Iran.
Sci Rep. 2023 Mar 6;13(1):3721. doi: 10.1038/s41598-023-30847-x.
Myocardial infarction (MI) affects many molecular pathways in heart cells, including the Ido1-KYN-Ahr axis. This pathway has recently been introduced as a valuable therapeutic target in infarction. We examined the effects of moderate-intensity continuous training (MICT) and high-intensity interval training (HIIT) on the axis in the heart tissue of male Wistar rats with occluded left anterior descending (OLAD). Thirty rats (age 10-12 weeks, mean weight 275 ± 25 g) were divided into five groups with 6 animals: Control (Ct) group, MICT group, rats with OLAD as MI group, rats with OLAD treated with MICT (MIMCT group) and rats with OLAD treated with HIIT (MIHIIT group). Rats performed the training protocols for 8 weeks, 5 days a week. HIIT included 7 sets of 4 min running with an intensity of 85-90% VOmax and 3 min of recovery activation between sets. MICT included continuous running at the same distance as HIIT with an intensity of 50-60% VOmax for 50 min. The expressions of Ahr, Cyp1a1, and Ido1 were assayed by real-time PCR. Malondialdehyde (MDA) and Kynurenine levels, and AHR, CYP1A1, and IDO1 proteins were detected using ELISA. Data were analyzed using the ANOVA and MANOVA tests. Compared to the CT group, MI caused an increase in all studied factors, but only statistically significant (P < 0.05) for MDA and IDO1. With a greater effect of HIIT, both protocols significantly lowered the proteins expressions in the MIHIIT and MIMCT groups, compared with the MI group (P < 0.001). In healthy rats, only AHR protein significantly decreased in the MICT group compared to the Ct group (P < 0.05). HIIT and MICT protocols significantly reduced the gene and protein expression of Cyp1a1 (P < 0.05) and Ido1 (P < 0.01), and HIIT had a greater effect. In conclusion, both protocols were effective at reducing the levels of Ido1-Kyn-Ahr axis components and oxidative stress in the infarcted heart tissue and HIIT had a higher significant effect.
心肌梗死（MI）影响心脏细胞中的许多分子途径，包括 IDO1-KYN-Ahr 轴。该途径最近被引入作为梗塞的有价值的治疗靶点。我们研究了中等强度连续训练（MICT）和高强度间歇训练（HIIT）对左前降支闭塞（OLAD）雄性 Wistar 大鼠心脏组织中该轴的影响。30 只大鼠（年龄 10-12 周，平均体重 275±25g）分为 5 组，每组 6 只：对照组（Ct）、MICT 组、MI 组、OLAD 治疗的 MICT 组（MIMCT 组）和 OLAD 治疗的 HIIT 组（MIHIIT 组）。大鼠进行 8 周的训练方案，每周 5 天。HIIT 包括 7 组 4 分钟的跑步，强度为 85-90% VOmax，每组之间恢复激活 3 分钟。MICT 包括与 HIIT 相同距离的连续跑步，强度为 50-60% VOmax，持续 50 分钟。通过实时 PCR 测定 Ahr、Cyp1a1 和 Ido1 的表达。使用 ELISA 检测丙二醛（MDA）和犬尿氨酸水平以及 AHR、CYP1A1 和 IDO1 蛋白。使用 ANOVA 和 MANOVA 测试分析数据。与 CT 组相比，MI 导致所有研究因素增加，但只有 MDA 和 IDO1 具有统计学意义（P<0.05）。由于 HIIT 的效果更大，两种方案都显著降低了 MIHIIT 和 MIMCT 组中的蛋白质表达，与 MI 组相比（P<0.001）。在健康大鼠中，只有 MICT 组的 AHR 蛋白与 Ct 组相比显著降低（P<0.05）。HIIT 和 MICT 方案显著降低 Cyp1a1（P<0.05）和 Ido1（P<0.01）的基因和蛋白表达，并且 HIIT 的效果更高。总之，两种方案都能有效降低梗塞心肌组织中 IDO1-Kyn-Ahr 轴成分和氧化应激的水平，而 HIIT 的效果更高。
