高强度训练预处理对雄性大鼠急性心肌梗死后组织中 G-CSF、其受体和 C-kit 水平的影响。
The effect of preconditioning with high-intensity training on tissue levels of G-CSF, its receptor and C-kit after an acute myocardial infarction in male rats.
机构信息
Department of Exercise physiology, Faculty of Physical Education and Sport Sciences, Kharazmi University, Tehran, Iran.
Physiology Research Center and Physiology Department, Faculty of Medicine, Iran University of Medical Sciences, Tehran, Iran.
出版信息
BMC Cardiovasc Disord. 2020 Feb 11;20(1):75. doi: 10.1186/s12872-020-01380-w.
BACKGROUND
Exercise training is known as a practical way to increase cardioprotection against stress, and it seems that stem cell recruitment is one of its mechanisms. The purpose of the present study was to investigate the effect of preconditioning with High-intensity interval training (HIIT) on tissue levels of G-CSF, its receptor and C-Kit following acute myocardial infarction in male rats.
METHODS
Twenty Male Wistar rats were randomly divided into 4 groups of control, MI, HIIT, and HIIT+MI. Training groups performed 2 weeks of high intensity interval training in 4 sections. The first section consisted training in 3 days and 2 sessions in each day (4 × 2 min with 35-40 m/min and 3 × 2 min with 25-30 m/min between high intervals. The second part included 2 days of training (4 × 2 min with 40 to 45 m/min and 3 × 2 min with 28 to 32 m /min). The third part was performed in 3 days with one more repetition. The fourth section consisted 2 days of training and with one more repetition compared to section 3. For induction of myocardial infarction, subcutaneous injection of isoprenaline was used. CK, total CK, LDH, and troponin T were measured in serum and G-CSF, G-CSFR and C-Kit proteins were measured by the Western Blot method in the heart tissue.
RESULTS
The results of this study showed that enzymes of CK, total CK, LDH, troponin T had a significant increase in both MI and HIIT+MI groups compared to the other two groups (P < 0.001) and these indices in the MI group were significantly higher than the HIIT+MI group. Also, the results demonstrated that G-CSF, G-CSFR and C-Kit protein expression in the heart tissue significantly increased after MI. As well as, 2 weeks of HIIT training significantly increased G-CSF and C-kit in the training group compared to the control group, but the training caused that these proteins does not increase in HIIT+MI group as much as MI group.
CONCLUSIONS
Along with other protective pathways, high intensity interval training can increase cardioprotection and decrease heart injuries through the increase in G-CSF, G-CSFR and C-kit level.
背景
运动训练被认为是增加应激性心脏保护的一种实用方法，而干细胞募集似乎是其机制之一。本研究的目的是探讨高强度间歇训练（HIIT）预处理对雄性大鼠急性心肌梗死后组织中 G-CSF、其受体和 C-Kit 水平的影响。
方法
将 20 只雄性 Wistar 大鼠随机分为对照组、MI 组、HIIT 组和 HIIT+MI 组。训练组进行了 2 周的高强度间歇训练，分为 4 节。第一节包括 3 天训练，每天 2 个疗程（4×2 分钟，间隔 35-40m/min，3×2 分钟，间隔 25-30m/min 之间进行高强度间隔）。第二节包括 2 天训练（4×2 分钟，速度为 40 到 45m/min，3×2 分钟，速度为 28 到 32m/min）。第三节在 3 天内进行了一次重复。第四节与第三节相比，进行了两天训练和一次重复。通过皮下注射异丙肾上腺素诱导心肌梗死。血清中测量 CK、总 CK、LDH 和肌钙蛋白 T，用 Western Blot 法测量心脏组织中的 G-CSF、G-CSFR 和 C-Kit 蛋白。
结果
本研究结果表明，与其他两组相比，MI 和 HIIT+MI 组的 CK、总 CK、LDH、肌钙蛋白 T 等酶均显著升高（P<0.001），且 MI 组明显高于 HIIT+MI 组。此外，结果表明，MI 后心脏组织中 G-CSF、G-CSFR 和 C-Kit 蛋白表达显著增加。同时，2 周的 HIIT 训练使训练组的 G-CSF 和 C-kit 明显高于对照组，但训练组使这些蛋白在 HIIT+MI 组中的增加不如 MI 组多。
结论
与其他保护途径一样，高强度间歇训练通过增加 G-CSF、G-CSFR 和 C-kit 水平，可增加心脏保护，减少心脏损伤。
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