Liu Minghui, Weng Jintao, Huang Shumei, Yin Wenjin, Zhang Huatang, Jiang Yin, Yang Liu, Sun Hongyan
School of Chemical Engineering and Light Industry and School of Biomedical and Pharmaceutical Sciences, Guangdong University of Technology, Guangzhou, Guangdong, 510006, China.
College of Chemistry and Chemical Engineering, Central South University, Changsha, Hunan, 410083, P. R. China.
Chem Commun (Camb). 2023 Mar 21;59(24):3570-3573. doi: 10.1039/d3cc00359k.
Lysosomal viscosity is a significant parameter of lysosomes and closely related to various diseases. Herein, two fluorescent probes, Lyso-vis-A and Lyso-vis-B, were developed, which demonstrate diverse advantages, including great water solubility, lysosome targeting ability and viscosity sensitivity. In particular, Lyso-vis-A exclusively showed fluorescence response toward viscosity but was not influenced by pH changes, rendering it a selective lysosomal viscosity probe. Furthermore, Lyso-vis-A was successfully applied to monitor lysosomal viscosity variations in living cells and differentiate cancer cells and normal cells.
溶酶体粘度是溶酶体的一个重要参数，与多种疾病密切相关。在此，开发了两种荧光探针Lyso-vis-A和Lyso-vis-B，它们具有多种优点，包括高水溶性、溶酶体靶向能力和粘度敏感性。特别地，Lyso-vis-A仅对粘度表现出荧光响应，不受pH变化的影响，使其成为一种选择性的溶酶体粘度探针。此外，Lyso-vis-A已成功应用于监测活细胞中溶酶体粘度的变化，并区分癌细胞和正常细胞。
