Baum P, Thorner J, Honig L
Proc Natl Acad Sci U S A. 1978 Oct;75(10):4962-6. doi: 10.1073/pnas.75.10.4962.
A tubulin-like protein was identified in the lower eukaryote Saccharomyces cerevisiae. The following criteria were used: (i) copolymerization of the 35S-labeled yeast protein with porcine brain tubulin; (ii) immunoprecipitation of the 35S-labeled yeast protein with antiflagellar tubulin antibody; (iii) the presence of the yeast protein as a constituent of isolated yeast nuclei; and (iv) splitting of the yeast protein in a gel electrophoretic system containing sodium dodecyl sulfate that resolved the alpha- and beta-tubulin chains from other sources. This protein did not appear to have significant affinity for the plant alkaloid, Colcemid.
在低等真核生物酿酒酵母中鉴定出一种微管蛋白样蛋白质。采用了以下标准：（i）35S标记的酵母蛋白与猪脑微管蛋白的共聚合；（ii）用抗鞭毛微管蛋白抗体对35S标记的酵母蛋白进行免疫沉淀；（iii）酵母蛋白作为分离的酵母细胞核的组成成分存在；（iv）在含有十二烷基硫酸钠的凝胶电泳系统中酵母蛋白的裂解，该系统可将来自其他来源的α和β微管蛋白链分开。这种蛋白质似乎对植物生物碱秋水仙酰胺没有显著亲和力。
