利用幼体斑马鱼快速检测脊髓损伤后轴突再生中的基因功能。
Rapid Testing of Gene Function in Axonal Regeneration After Spinal Cord Injury Using Larval Zebrafish.
机构信息
Centre for Discovery Brain Sciences, University of Edinburgh, Edinburgh, UK.
Center for Regenerative Therapies Dresden (CRTD), Technische Universität Dresden, Dresden, Germany.
出版信息
Methods Mol Biol. 2023;2636:263-277. doi: 10.1007/978-1-0716-3012-9_15.
PMID:36881306
Abstract
Larval zebrafish show axonal regrowth over a complex spinal injury site and recovery of function within days after injury. Here we describe a simple protocol to disrupt gene function in this model using acute injections of highly active synthetic gRNAs to rapidly detect loss-of-function phenotypes without the need for breeding.
摘要
幼体斑马鱼在复杂的脊髓损伤部位表现出轴突再生，并在损伤后数天内恢复功能。在这里，我们描述了一种简单的方案，通过急性注射高效合成的 gRNA 来破坏该模型中的基因功能，无需繁殖即可快速检测到功能丧失表型。
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