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密度梯度离心法、水平上游法扩展版以及两者结合用于精子筛选的比较。

Comparison between density gradient centrifugation method, an extended version of the horizontal swim up method and the combination of both for sperm selection.

作者信息

Jamil Malak, Debbarh Hasnae, Kabit Amal, Ennaji Mohamed, Koumba Loic, Kaarouch Ismail, Zarqaoui Mohamed, Senhaji Wassim Rhazi, Hissane El Mehdi, Saadani Brahim, Vanderzwalmen Pierre, Louanjli Nourredine, Cadi Rachida

机构信息

Department of Biology, Laboratory of Molecular Genetic Physiopathology and Biotechnology, Ain Chock Faculty of Sciences, HASSAN II University Casablanca, Morocco.

Labomac IVF Centers and Clinical Laboratory Medicine, Casablanca, Morocco.

出版信息

Obstet Gynecol Sci. 2023 May;66(3):221-229. doi: 10.5468/ogs.22191. Epub 2023 Mar 8.

DOI:10.5468/ogs.22191
PMID:36883228
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10191761/
Abstract

OBJECTIVE

To compare the degree of efficiency between density gradient centrifugation (DGC) method and an extended horizontal swim-up (SU) method.

METHODS

A total of 97 couples undergoing in vitro fertilization were enrolled in the study. Semen samples were divided into three aliquots and treated using DGC, extended horizontal SU, and combined methods. DNA fragmentation and chromatin decondensation were detected in native semen samples and their three corresponding aliquots. The corresponding mature oocytes of each semen sample were divided into two sibling cultures. The first sibling culture was microinjected with semen pellets from DGC, and the second sibling culture was microinjected with semen pellets from the combination of both methods. Fertilization rate and embryonic development were assessed at day 3.

RESULTS

DNA fragmentation and chromatin decondensation was significantly low in DGC and extended horizontal SU samples; however, the rates of DNA fragmentation and chromatin decondensation were significantly lower in extended horizontal SU samples than in DGC samples. The lowest rates of DNA fragmentation and chromatin decondensation corresponded to the samples treated with both methods. The highest rates of DNA fragmentation and chromatin decondensation corresponded to the samples treated with DGC. No significant difference was found in the fertilization rate or day 3 embryos between sibling cultures.

CONCLUSION

The combination of DGC and the extended horizontal SU techniques is best for giving the lowest rates of sperm DNA fragmentation and chromatin decondensation.

摘要

目的

比较密度梯度离心（DGC）法与改良水平上游（SU）法的效率。

方法

共纳入97对接受体外受精的夫妇。精液样本分为三等份，分别采用DGC法、改良水平SU法及联合方法处理。对原精液样本及其三个相应的等分样本进行DNA片段化和染色质解聚检测。每个精液样本对应的成熟卵母细胞分为两个平行培养组。第一个平行培养组用DGC法获得的精液颗粒进行显微注射，第二个平行培养组用两种方法联合获得的精液颗粒进行显微注射。在第3天评估受精率和胚胎发育情况。

结果

DGC法和改良水平SU法样本中的DNA片段化和染色质解聚程度显著较低；然而，改良水平SU法样本中的DNA片段化率和染色质解聚率显著低于DGC法样本。DNA片段化和染色质解聚率最低的样本对应两种方法联合处理的样本。DNA片段化和染色质解聚率最高的样本对应DGC法处理的样本。平行培养组之间的受精率或第3天胚胎情况未发现显著差异。

结论

DGC法与改良水平SU技术联合使用最有利于使精子DNA片段化和染色质解聚率降至最低。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/abb2/10191761/fe858a38ab2f/ogs-22191f2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/abb2/10191761/bd8db2ca314d/ogs-22191f1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/abb2/10191761/fe858a38ab2f/ogs-22191f2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/abb2/10191761/bd8db2ca314d/ogs-22191f1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/abb2/10191761/fe858a38ab2f/ogs-22191f2.jpg

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