Wachsman M B, Damonte E B, Coto C E, de Torres R A
Laboratorio de Virología, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Argentina.
Antiviral Res. 1987 Aug;8(1):1-12. doi: 10.1016/0166-3542(87)90083-0.
Partially purified extracts from leaves of Melia azedarach L. (MA) exert a broad range of antiviral effects on DNA and RNA viruses. The effect of MA on different stages of Sindbis virus replicative cycle in BHK cells was investigated. Under one-step growth conditions MA afforded a greater than 90% inhibition in virus yield if added to the cell cultures 2 h before or after infection, and when added 4 h after infection MA still caused a greater than 80% inhibition. Analysis of early events following Sindbis virus infection showed that MA did not affect viral adsorption to or penetration in BHK cell. In contrast, viral RNA and protein synthesis was almost totally inhibited in cells pretreated with MA 2 h before infection, while cellular macromolecular synthesis was similar in MA-treated and untreated cell cultures.
苦楝叶的部分纯化提取物对DNA和RNA病毒具有广泛的抗病毒作用。研究了苦楝提取物对辛德毕斯病毒在BHK细胞中复制周期不同阶段的影响。在一步生长条件下，如果在感染前2小时或感染后添加到细胞培养物中，苦楝提取物对病毒产量的抑制率大于90%；当在感染后4小时添加时，苦楝提取物仍能产生大于80%的抑制作用。对辛德毕斯病毒感染后早期事件的分析表明，苦楝提取物不影响病毒对BHK细胞的吸附或穿透。相反，在感染前2小时用苦楝提取物预处理的细胞中，病毒RNA和蛋白质合成几乎完全受到抑制，而在苦楝提取物处理和未处理的细胞培养物中，细胞大分子合成相似。
