Pioli KimAnh T, Lau Kin H, Pioli Peter D
Department of Biochemistry, Microbiology and Immunology, College of Medicine, University of Saskatchewan, Saskatoon, SK S7N5E5, Canada.
Bioinformatics and Biostatistics Core, Van Andel Institute, Grand Rapids, MI 49503, USA.
iScience. 2023 Feb 15;26(3):106223. doi: 10.1016/j.isci.2023.106223. eCollection 2023 Mar 17.
Antibody-secreting cells (ASCs) are key contributors to humoral immunity through immunoglobulin production and the potential to be long-lived. ASC persistence has been recognized in the autoimmune thymus (THY); however, only recently has this population been appreciated in healthy THY tissue. We showed that the young female THY was skewed toward higher production of ASCs relative to males. However, these differences disappeared with age. In both sexes, THY ASCs included Ki-67 plasmablasts which required CD154(CD40L) signals for their propagation. Single cell RNA-sequencing revealed that THY ASCs were enriched for an interferon responsive transcriptional signature relative to those from bone marrow and spleen. Flow cytometry confirmed that THY ASCs had increased levels of Toll-like receptor 7 as well as CD69 and major histocompatibility complex class II. Overall, we identified fundamental aspects of THY ASC biology which may be leveraged for future in depth studies of this population in both health and disease.
抗体分泌细胞（ASC）通过产生免疫球蛋白以及具有长期存活的潜力，成为体液免疫的关键贡献者。ASC在自身免疫性胸腺（THY）中的持续存在已得到认可；然而，直到最近才在健康的THY组织中认识到这一群体。我们发现，相对于雄性，年轻雌性THY倾向于产生更多的ASC。然而，这些差异随着年龄的增长而消失。在两性中，THY ASC包括Ki-67浆母细胞，其增殖需要CD154（CD40L）信号。单细胞RNA测序显示，相对于来自骨髓和脾脏的ASC，THY ASC富含干扰素反应性转录特征。流式细胞术证实，THY ASC的Toll样受体7以及CD69和主要组织相容性复合体II类的水平有所增加。总体而言，我们确定了THY ASC生物学的基本方面，这可能有助于未来对该群体在健康和疾病中的深入研究。
