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拟南芥根中（）和（）基因表达对铵供应的共调节。 （注：原文括号部分内容缺失）

Coregulation of () and () gene expression in Arabidopsis roots in response to ammonium supply.

作者信息

Kojima Soichi, Minagawa Haruka, Yoshida Chika, Inoue Eri, Takahashi Hideki, Ishiyama Keiki

机构信息

Graduate School of Agricultural Science, Tohoku University, Sendai, Japan.

Plant Science Center, RIKEN, Yokohama, Japan.

出版信息

Front Plant Sci. 2023 Feb 20;14:1127006. doi: 10.3389/fpls.2023.1127006. eCollection 2023.

DOI:10.3389/fpls.2023.1127006
PMID:36890884
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9986259/
Abstract

Ammonium absorbed by roots is assimilated into amino acids. The glutamine synthetase/glutamate synthase (glutamine 2-oxoglutarate aminotransferase) (GS/GOGAT) cycle is essential to this biological process. In , and are the and isoenzymes induced in response to ammonium supply and playing key roles in ammonium utilization. Although recent studies suggest gene regulatory networks involved in transcriptional regulation of ammonium-responsive genes, direct regulatory mechanisms for ammonium-induced expression of remain unclear. In this study, we revealed that the expression of and in Arabidopsis is not directly induced by ammonium but is regulated by glutamine or post-glutamine metabolites produced by ammonium assimilation. Previously, we identified a promoter region required for ammonium-responsive expression of . In this study, we further dissected the ammonium-responsive region of the promoter and also performed a deletion analysis of the promoter, which led to the identification of a conserved ammonium-responsive region. Yeast one-hybrid screening using the ammonium-responsive region of the promoter as a decoy sequence revealed a trihelix family transcription factor DF1 that binds to this region. A putative DF1 binding site was also found in the ammonium-responsive region of the promoter.

摘要

根系吸收的铵被同化为氨基酸。谷氨酰胺合成酶/谷氨酸合酶（谷氨酰胺-2-氧代戊二酸氨基转移酶）（GS/GOGAT）循环对这一生物学过程至关重要。在[具体内容缺失]中，[具体内容缺失]和[具体内容缺失]是响应铵供应而诱导产生的[具体内容缺失]和[具体内容缺失]同工酶，在铵利用中起关键作用。尽管最近的研究表明存在参与铵响应基因转录调控的基因调控网络，但铵诱导[具体基因缺失]表达的直接调控机制仍不清楚。在本研究中，我们发现拟南芥中[具体基因缺失]和[具体基因缺失]的表达并非由铵直接诱导，而是受铵同化产生的谷氨酰胺或谷氨酰胺后代谢产物调控。此前，我们鉴定了[具体基因缺失]铵响应表达所需的启动子区域。在本研究中，我们进一步剖析了[具体基因缺失]启动子的铵响应区域，并对[具体基因缺失]启动子进行了缺失分析，从而鉴定出一个保守的铵响应区域。以[具体基因缺失]启动子的铵响应区域为诱饵序列进行酵母单杂交筛选，发现了一个与该区域结合的三螺旋家族转录因子DF1。在[具体基因缺失]启动子的铵响应区域也发现了一个假定的DF1结合位点。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b44c/9986259/75fd214ad9ef/fpls-14-1127006-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b44c/9986259/45c1dbdfd046/fpls-14-1127006-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b44c/9986259/32f7618f4f0c/fpls-14-1127006-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b44c/9986259/4d44f51aa628/fpls-14-1127006-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b44c/9986259/09ad5f1ce6a3/fpls-14-1127006-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b44c/9986259/75fd214ad9ef/fpls-14-1127006-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b44c/9986259/45c1dbdfd046/fpls-14-1127006-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b44c/9986259/32f7618f4f0c/fpls-14-1127006-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b44c/9986259/4d44f51aa628/fpls-14-1127006-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b44c/9986259/09ad5f1ce6a3/fpls-14-1127006-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b44c/9986259/75fd214ad9ef/fpls-14-1127006-g005.jpg

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