依赖烟酰胺腺嘌呤二核苷酸（NADH）的谷氨酸合酶参与拟南芥根中的铵同化作用。
NADH-dependent glutamate synthase participated in ammonium assimilation in Arabidopsis root.
作者信息
Kojima Soichi, Konishi Noriyuki, Beier Marcel Pascal, Ishiyama Keiki, Maru Ikumi, Hayakawa Toshihiko, Yamaya Tomoyuki
机构信息
a Graduate School of Agricultural Science; Tohoku University; Sendai, Japan.
出版信息
Plant Signal Behav. 2014;9(8):e29402. doi: 10.4161/psb.29402.
Higher plants have 2 GOGAT species, Fd-GOGAT and NADH-GOGAT. While Fd-GOGAT mainly assimilates ammonium in leaves, which is derived from photorespiration, the function of NADH-GOGAT, which is highly expressed in roots, (1) needs to be elucidated. The aim of this study was to clarify the role of NADH-GOGAT in Arabidopsis roots. The supply of ammonium to the roots caused an accumulation of NADH-GOGAT, while Fd-GOGAT 1 and Fd-GOGAT 2 showed no response. A promoter-GUS fusion analysis and immunohistochemistry showed that NADH-GOGAT was located in non-green tissues like vascular bundles, shoot apical meristem, pollen, stigma, and roots. The localization of NADH-GOGAT and Fd-GOGAT was not overlapped. NADH-GOGAT T-DNA insertion lines showed a reduction of glutamate and biomass under normal CO2 conditions. These data emphasizes the importance of NADH-GOGAT in the ammonium assimilation of Arabidopsis roots.
高等植物有两种谷氨酰胺合成酶/谷氨酸合酶（GOGAT），即铁氧还蛋白-GOGAT和NADH-GOGAT。虽然铁氧还蛋白-GOGAT主要在叶片中同化铵，该铵来源于光呼吸，但在根中高度表达的NADH-GOGAT的功能（1）仍有待阐明。本研究的目的是明确NADH-GOGAT在拟南芥根中的作用。向根供应铵会导致NADH-GOGAT积累，而铁氧还蛋白-GOGAT 1和铁氧还蛋白-GOGAT 2则无反应。启动子-GUS融合分析和免疫组织化学表明，NADH-GOGAT位于维管束、茎尖分生组织、花粉、柱头和根等非绿色组织中。NADH-GOGAT和铁氧还蛋白-GOGAT的定位不重叠。NADH-GOGAT T-DNA插入系在正常二氧化碳条件下显示谷氨酸和生物量减少。这些数据强调了NADH-GOGAT在拟南芥根铵同化中的重要性。
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