Food Technology Department, Arid Land Cultivation Research Institute, City of Scientific Research and Technological Applications, New Borg El-Arab City, P.O. Box 21934 Alexandria, Egypt.
Protein Research Department, Genetic Engineering and Biotechnology Research Institute (GEBRI), City of Scientific Research and Technological Applications (SRTA-City), New Borg El-Arab City, P.O. Box 21934 Alexandria, Egypt.
ScientificWorldJournal. 2023 Feb 27;2023:9919814. doi: 10.1155/2023/9919814. eCollection 2023.
In this study, previously isolated from Lake Mariout (Alexandria, Egypt) was cultivated in the laboratory using a medium for pharmaceutical grade , named as Amara and Steinbüchel medium. Hot water extract of the Egyptian was prepared by autoclaving dried biomass in distilled water at 121°C for 15 min. This algal water extract was analyzed by GC-MS to evaluate its volatile compounds and fatty acids composition. The antimicrobial activity of phycobiliprotein extract from using phosphate buffer was evaluated against thirteen microbial strains (two Gram-positive bacteria, eight Gram-negative bacteria, one yeast, and two filamentous fungi). The major components of fatty acids in the hot extract of Egyptian were hexadecanoic acid (palmitic acid, 55.19%) and octadecanoic acid (stearic acid, 27.14%). The main constituents of its volatile compounds were acetic acid (43.33%) and oxalic acid (47.98%). The most potent antimicrobial effect of phycobiliprotein extract was obtained against two Gram-negative bacteria and , filamentous fungus , and the pathogenic yeast (all of which showed MIC values of 58.1 g/ml). and come second in their susceptibility to the phycobiliprotein extract from and and are the least in susceptibility, with MIC values of 116.2 and 232.5 g/ml, respectively, while phycobiliprotein extract has no antibacterial effect on methicillin-resistant as well as susceptible , , , and . These findings confirmed the nutritional value of Egyptian isolated from Lake Mariout and suggest the potential use of this strain as an ingredient in the cooking of some foods to increase the level of stearic acid and palmitic acid. Moreover, its effective antibacterial activities against some important and highly resistant to antibiotics bacterial pathogens in addition to its antifungal effects recommend the therapeutic use of its biomass.
在这项研究中，先前从埃及亚历山大的 Mariout 湖分离出的蓝藻被在实验室中使用一种名为 Amara 和 Steinbüchel 的制药级培养基进行培养。通过在 121°C 下将干燥的生物质在蒸馏水中高压灭菌 15 分钟来制备埃及蓝藻的热水提取物。通过 GC-MS 分析该藻类水提取物，以评估其挥发性化合物和脂肪酸组成。使用磷酸盐缓冲液从蓝藻藻蓝蛋白提取物的抗菌活性评价了其对 13 种微生物菌株（两种革兰氏阳性菌、八种革兰氏阴性菌、一种酵母和两种丝状真菌）的影响。埃及蓝藻热水提取物中脂肪酸的主要成分是十六烷酸（棕榈酸，55.19%）和十八烷酸（硬脂酸，27.14%）。其挥发性化合物的主要成分是乙酸（43.33%）和草酸（47.98%）。藻蓝蛋白提取物的最强抗菌效果是针对两种革兰氏阴性菌 和 、丝状真菌 和致病性酵母 （均显示 MIC 值为 58.1μg/ml）。 和 对藻蓝蛋白提取物的敏感性次之， 和 对藻蓝蛋白提取物的敏感性最低，MIC 值分别为 116.2 和 232.5μg/ml，而藻蓝蛋白提取物对耐甲氧西林的 以及 、 、 和 没有抗菌作用。这些发现证实了从 Mariout 湖分离出的埃及蓝藻的营养价值，并表明该菌株可能作为某些食品烹饪的成分，以增加硬脂酸和棕榈酸的含量。此外，其对一些重要的、对抗生素高度耐药的细菌病原体的有效抗菌活性，以及其抗真菌作用，推荐其生物质的治疗用途。
