Liao Weifang, Duan Xunxin, Xie Fusheng, Zheng Dongxi, Yang Pu, Wang Xiangguo, Hu Zhijian
Department of Medical Laboratory, Affiliated Hospital of Jiujiang University, China; Jiujiang Clinical Precision Medicine Research Center, Jiujiang, Jiangxi, China; Jiangxi Provincial Clinical Research Center for Laboratory Medicine, Nanchang, Jiangxi, China.
School of Mechanical and Intelligent Manufacturing, Jiujiang University, Jiujiang, Jiangxi, China.
Int J Biol Macromol. 2023 May 1;236:123952. doi: 10.1016/j.ijbiomac.2023.123952. Epub 2023 Mar 8.
Improving chronic wound healing remains a challenge in the clinical practice. In this study, we developed double-crosslinked angiogenic 3D-bioprinted patches for diabetic wound healing by the photocovalent crosslinking of vascular endothelial growth factor (VEGF) using ultraviolet (UV) irradiation. 3D printing technology can precisely customize the structure and composition of patches to meet different clinical requirements. The biological polysaccharide alginate and chondroitin sulfate methacryloyl were used as biomaterials to construct the biological patch, which could be crosslinked using calcium ion crosslinking and photocrosslinking, thereby improving its mechanical properties. More importantly, acrylylated VEGF could be easily and rapidly photocrosslinked under UV irradiation, which simplified the step of chemically coupling growth factors and prolonged VEGF release time. These characteristics suggest that 3D-bioprinted double-crosslinked angiogenic patches are ideal candidates for diabetic wound healing and other tissue engineering applications.
在临床实践中，改善慢性伤口愈合仍然是一项挑战。在本研究中，我们通过使用紫外线（UV）照射对血管内皮生长因子（VEGF）进行光共价交联，开发了用于糖尿病伤口愈合的双交联血管生成3D生物打印贴片。3D打印技术可以精确定制贴片的结构和组成，以满足不同的临床需求。生物多糖藻酸盐和甲基丙烯酰化硫酸软骨素被用作生物材料来构建生物贴片，该贴片可以通过钙离子交联和光交联进行交联，从而改善其机械性能。更重要的是，丙烯酰化VEGF在紫外线照射下可以轻松快速地进行光交联，这简化了生长因子化学偶联的步骤并延长了VEGF的释放时间。这些特性表明，3D生物打印双交联血管生成贴片是糖尿病伤口愈合和其他组织工程应用的理想候选者。
