使用LKB 2086反应速率分析仪通过EMIT法测定血清中抗癫痫药物的研究。

Study of the EMIT method for determination of anti-epileptic drugs in serum using an LKB 2086 reaction rate analyzer.

作者信息

Christensen N J

出版信息

Scand J Clin Lab Invest. 1978 Dec;38(8):781-4. doi: 10.1080/00365517809104888.

Abstract

Phenemal, phenytoin and carbamazepine were determined by the EMIT method on an LKB 2086 Reaction Rate Analyzer and the results compared with routine results obtained for phenemal by gas chromatography and for phenytoin and carbamazepine by thin layer chromatography. Coefficients of variation for phenemal at the concentration 99 mumol/l in bovine serum were 3.1% within run and 10.8% between run. For phenytoin the coefficients of variation were respectively 13.1% and 15.4% for the same serum with a concentration of 99 mumol/l. For carbamazepine the coefficients of variation were estimated to be 14.1% within run and 10.4% between run for a human serum containing 25 mumol/l. With an LKB 2082 Kinetic Data System the coefficients of variation phenytoin and carbamazepine were reduced to 8% and the coefficients of correlation between the EMIT methods and the routine methods were at least 0.965 for all three drugs. The assay ranges were found to be satisfactory. The method allows assay down to 8.6, 4.0 and 2.1 mumol/l and up to 301, 198, and 51 mumol/l for phenemal, phenytoine and carbamazepine, respectively.

摘要

采用酶放大免疫测定技术（EMIT）在LKB 2086反应速率分析仪上测定苯巴比妥、苯妥英和卡马西平，并将结果与通过气相色谱法测定苯巴比妥以及通过薄层色谱法测定苯妥英和卡马西平所获得的常规结果进行比较。在牛血清中浓度为99 μmol/l时，苯巴比妥的批内变异系数为3.1%，批间变异系数为10.8%。对于浓度为99 μmol/l的同一份血清，苯妥英的变异系数分别为13.1%和15.4%。对于含有25 μmol/l的人血清，卡马西平的批内变异系数估计为14.1%，批间变异系数为10.4%。使用LKB 2082动力学数据系统时，苯妥英和卡马西平的变异系数降至8%，并且三种药物的EMIT方法与常规方法之间的相关系数至少为0.965。检测范围令人满意。该方法对苯巴比妥、苯妥英和卡马西平的检测下限分别可达8.6、4.0和2.1 μmol/l，上限分别可达301、198和51 μmol/l。