Kuang Jingyu, Ju Jiahe, Lu Yongli, Chen Yitong, Liu Chang, Kong Dezhao, Shen Wei, Shi Hai-Wei, Li Li, Ye Jin, Tang Sheng
School of Environment and Chemical Engineering, Jiangsu University of Science and Technology, Zhenjiang 212003, Jiangsu Province, PR China.
School of Grain Science and Technology, Jiangsu University of Science and Technology, Zhenjiang 212003, Jiangsu Province, PR China.
Food Chem. 2023 Aug 1;416:135856. doi: 10.1016/j.foodchem.2023.135856. Epub 2023 Mar 4.
In this work, a highly sensitive method for aflatoxin B (AFB) detection was developed based on a peroxidase-like spatial network structure. The specific antibody and antigen of AFB were coated on a histidine-modified FeO nanozyme to form the capture/detection probes. Based on the competition/affinity effect, the spatial network structure was constructed by the probes, which could be rapidly (8 s) separated by a magnetic three-phase single-drop microextraction process. In this single-drop microreactor, the network structure was applied to catalyze a colorimetric 3,3',5,5'-tetramethylbenzidine oxidation reaction for AFB detection. The signal was amplified significantly due to the strong peroxidase-like ability of the spatial network structure and the enrichment effect of the microextraction. Thus, a low detection limit (0.034 pg/mL) was achieved. The matrix effect of real sample can be eliminated by the extraction approach, and the practicability of this method was proved by agricultural product samples analysis.
在本研究中，基于类过氧化物酶空间网络结构开发了一种高灵敏度的黄曲霉毒素B（AFB）检测方法。将AFB的特异性抗体和抗原包被在组氨酸修饰的FeO纳米酶上，形成捕获/检测探针。基于竞争/亲和效应，由探针构建空间网络结构，该结构可通过磁性三相单滴微萃取过程快速（8秒）分离。在这个单滴微反应器中，该网络结构用于催化比色法检测AFB的3,3',5,5'-四甲基联苯胺氧化反应。由于空间网络结构强大的类过氧化物酶能力和微萃取的富集作用，信号得到显著放大。因此，实现了低检测限（0.034 pg/mL）。通过萃取方法可消除实际样品的基质效应，农产品样品分析证明了该方法的实用性。
