Wei Dan, Li Jianliang, Zheng Shuangshuang, Guo Ming, Xu Jingjing, Deng Qiao, Wang Xu
Hangzhou Medical College, School of Laboratory Medicine and Bioengineering, Hangzhou, 310053, China, Zhejiang 311300, China.
Key Laboratory of Biomarkers and In Vitro Diagnosis Translation of Zhejiang province, Hangzhou 310058, China.
Food Chem X. 2024 Aug 31;24:101797. doi: 10.1016/j.fochx.2024.101797. eCollection 2024 Dec 30.
Cereals are frequently contaminated by aflatoxins (AFs). The objective of this study was to develop an efficient extraction materials for rapidly extracting and detecting AFs. A novel amino-functionalized benzodiimidazole linkage magnetic covalent organic framework (FeO@BB-COF) was simply fabricated by one-step cyclization and aromatization. The FeO@BB-COF, having multiple N-containing active sites, exhibited excellent extraction capability towards AFs due to synergistic interactions, including the π-π interactions, hydrogen bonding interactions, polar interactions, electrostatic interactions and Lewis acid-base interactions. The FeO@BB-COF based MSPE method for detecting aflatoxins has advantages of simple operation, short extraction time (6 min), and low material consumption (2 mg). This method exhibited satisfactory linearity (0.05-20 μg/kg), and sensitivity (0.01-0.45 μg/L for the detection limits) and accuracy (76.8-97.1 % for recovery) and was successfully applied for extracting and detecting AFs in cereals.
谷物经常受到黄曲霉毒素（AFs）的污染。本研究的目的是开发一种高效的萃取材料，用于快速萃取和检测黄曲霉毒素。通过一步环化和芳构化简单制备了一种新型氨基功能化苯并二咪唑连接的磁性共价有机骨架（FeO@BB-COF）。FeO@BB-COF具有多个含氮活性位点，由于协同相互作用，包括π-π相互作用、氢键相互作用、极性相互作用、静电相互作用和路易斯酸碱相互作用，对黄曲霉毒素表现出优异的萃取能力。基于FeO@BB-COF的磁性固相萃取（MSPE）法检测黄曲霉毒素具有操作简单、萃取时间短（6分钟）和材料消耗低（2毫克）的优点。该方法具有令人满意的线性（0.05-20μg/kg）、灵敏度（检测限为0.01-0.45μg/L）和准确度（回收率为76.8-97.1%），并成功应用于谷物中黄曲霉毒素的萃取和检测。
