Zou Rongfang, Bi Lunan, Huang Yang, Wang Yadi, Wang Yan, Li Lin, Liu Jiayin, Feng Lu, Jiang Xiaoxia, Deng Bin
Chinese PLA Medical School, Beijing, 100853, China; Department of Stomatology, the First Medical Center, Chinese PLA General Hospital, Beijing, 100853, China.
Shandong Industrial Ceramic Research and Design Institute Co. Ltd., Zibo, 255000, Shandong, China.
J Mech Behav Biomed Mater. 2023 May;141:105756. doi: 10.1016/j.jmbbm.2023.105756. Epub 2023 Mar 4.
For decades, titanium has been the preferred material for dental implant fabrication. However, metallic ions and particles can cause hypersensitivity and aseptic loosening. The growing demand for metal-free dental restorations has also promoted the development of ceramic-based dental implants, such as silicon nitride. In this study, silicon nitride (SiN) dental implants were fabricated for biological engineering by photosensitive resin based digital light processing (DLP) technology, comparable to conventionally produced SiN ceramics. The flexural strength was (770 ± 35) MPa by the three-point bending method, and the fracture toughness was (13.3 ± 1.1) MPa · m by the unilateral pre-cracked beam method. The elastic modulus measured by the bending method was (236 ± 10) GPa. To confirm whether the prepared SiN ceramics possessed good biocompatibility, in vitro biological experiments were performed with the fibroblast cell line L-929, and preferable cell proliferation and apoptosis were observed at the initial stages. Hemolysis test, oral mucous membrane irritation test, and acute systemic toxicity test (oral route) further confirmed that the SiN ceramics did not exhibit hemolysis reaction, oral mucosal stimulation, or systemic toxicity. The findings indicate that SiN dental implant restorations with personalized structures prepared by DLP technology have good mechanical properties and biocompatibility, which has great application potential in the future.
几十年来，钛一直是牙种植体制造的首选材料。然而，金属离子和颗粒会导致过敏反应和无菌性松动。对无金属牙科修复体的需求不断增长，也推动了基于陶瓷的牙种植体的发展，如氮化硅。在本研究中，采用基于光敏树脂的数字光处理（DLP）技术制备了用于生物工程的氮化硅（SiN）牙种植体，其性能与传统生产的SiN陶瓷相当。通过三点弯曲法测得的抗弯强度为（770±35）MPa，通过单边预制裂纹梁法测得的断裂韧性为（13.3±1.1）MPa·m。通过弯曲法测得的弹性模量为（236±10）GPa。为了确认制备的SiN陶瓷是否具有良好的生物相容性，使用成纤维细胞系L-929进行了体外生物学实验，在初始阶段观察到了较好的细胞增殖和凋亡情况。溶血试验、口腔黏膜刺激试验和急性全身毒性试验（口服途径）进一步证实，SiN陶瓷未表现出溶血反应、口腔黏膜刺激或全身毒性。研究结果表明，通过DLP技术制备的具有个性化结构的SiN牙种植体修复体具有良好的力学性能和生物相容性，在未来具有巨大的应用潜力。
