Srivastava Neha, Singh Rajeev, Ahmad Irfan, Asiri Mohammed, Tripathi Subhash C, Rai Ashutosh Kumar, Mishra P K, Gupta Vijai Kumar
Department of Chemical Engineering & Technology, Indian Institute of Technology (BHU) Varanasi, Varanasi 221005, Uttar Pradesh, India.
Department of Environmental Science, Jamia Millia Islamia, (A Central University), New Delhi 110025, India.
Bioresour Technol. 2023 May;376:128847. doi: 10.1016/j.biortech.2023.128847. Epub 2023 Mar 9.
Due to the limited availability of fossil fuels, pollution causing serious environmental issues, and their continuously rising price, the development of low-cost efficient enzymes and their implementation in biomass-based bioenergy industries are highly demanded. In the present work, phytogenic fabrication of copper oxide based nanocatalyst has been performed using moringa leaves and has been characterized using different techniques. Herein, the impact of different dosages of as-prepared nanocatalyst on fungal co-cultured cellulolytic enzyme production under co-substrate fermentation using wheat straw and sugarcane bagasse in 4:2 ratios in solid state fermentation (SSF) has been investigated. An optimal concentration of 25 ppm of nanocatalyst influenced the production of 32 IU/gds of enzyme, which showed thermal stability at 70 °C for 15 h. Additionally, enzymatic bioconversion of rice husk at 70 °C librated 41 g/L of total reducing sugars, which led to the production of 2390 mL/L of cumulative H in 120 h.
由于化石燃料的供应有限、造成严重环境问题的污染以及其价格持续上涨，对低成本高效酶的开发及其在生物质基生物能源产业中的应用需求迫切。在本研究中，利用辣木叶进行了基于氧化铜的纳米催化剂的植物源制备，并采用不同技术对其进行了表征。在此，研究了在固态发酵（SSF）中，以4:2的比例使用小麦秸秆和甘蔗渣作为共底物发酵时，不同剂量的制备好的纳米催化剂对真菌共培养纤维素分解酶产生的影响。25 ppm的纳米催化剂最佳浓度影响了酶的产生，酶活为32 IU/gds，在70°C下15小时显示出热稳定性。此外，稻壳在70°C下的酶促生物转化释放出41 g/L的总还原糖，在120小时内产生了2390 mL/L的累积氢气。
