Department of Pathology, The Fourth Hospital of Hebei Medical University/Hebei Tumor Hospital, Shijiazhuang, China.
Health Examination Center, Langfang TCM Hospital, Langfang, China.
Bull Exp Biol Med. 2023 Feb;174(4):478-481. doi: 10.1007/s10517-023-05733-1. Epub 2023 Mar 10.
We studied the mechanism underlying the effect of baicalein on the growth of transplanted esophageal cancer in NOG mice and its effect on the expression of PAK4. For that purpose, we developed a new model of transplanted esophageal cancer (human esophageal cancer OE19 cells (10 cells/ml) were inoculated to NOG mice). Three experimental groups with transplanted esophageal cancer cells received baicalein in different doses (1, 1.5, and 2 mg/kg). In 32 days, the tumors were resected, and the expression of PAK4 and the level of activated PAK4 were assayed by reverse transcription PCR and Western blotting, respectively. The results showed a dose-depending anti-tumor effect of baicalein on the transplanted esophageal cancer in NOG mice: this effect of baicalein (determined by the size and weight of the tumor) increased with increasing the dose of the substance. Furthermore, the anti-tumor effect of baicalein was also confirmed by reduction of PAK4 expression. Thus, baicalein can inhibit tumor growth by inhibiting activation of PAK4. Therefore, our results showed that baicalein could inhibit the growth of esophageal cancer cells by inhibiting the activity of PAK4, which can be an important mechanism of its antitumor effect.
我们研究了黄芩素抑制 NOG 小鼠移植性食管癌生长及其对 PAK4 表达影响的作用机制。为此，我们建立了一种新的移植性食管癌模型（将 10 个/ml 的人食管癌细胞 OE19 接种到 NOG 小鼠中）。三组移植性食管癌细胞接受不同剂量的黄芩素（1、1.5 和 2mg/kg）。32 天后切除肿瘤，通过逆转录 PCR 和 Western blot 分别检测 PAK4 的表达和活化 PAK4 的水平。结果表明，黄芩素对 NOG 小鼠移植性食管癌具有剂量依赖性的抗肿瘤作用：这种黄芩素的作用（通过肿瘤的大小和重量来确定）随着物质剂量的增加而增加。此外，黄芩素还通过降低 PAK4 的表达来证实其抗肿瘤作用。因此，黄芩素可以通过抑制 PAK4 的激活来抑制肿瘤生长。因此，我们的研究结果表明，黄芩素通过抑制 PAK4 的活性抑制食管癌细胞的生长，这可能是其抗肿瘤作用的重要机制。
