Biosafety Research Institute, College of Veterinary Medicine, Chonbuk National University, Iksana, Republic of Korea.
CHA Bundang Medical Center, CHA University, Seongnam, Republic of Korea.
Oncol Rep. 2017 Nov;38(5):3011-3018. doi: 10.3892/or.2017.6007. Epub 2017 Sep 26.
Baicalein, a herbal medicine, is a natural flavonoid isolated from the roots of Scutellaria baicalensis Georgi. It is known for its anticancer, anti-inflammatory and neuroprotective properties. Despite these well-known properties, it is not yet clear what effect baicalein has on tumor progression. Therefore, in the present study, we used B16F10 cells, Lewis lung carcinoma (LLC) cells, and human umbilical vein endothelial cells (HUVECs) to investigate the effect of baicalein on cell proliferation and viability, migration and tube formation in vitro. In addition, an experimental animal model was used to observe the growth rate and metastasis of tumors and tumor vessel formation in vivo. Our results showed that baicalein decreased the proliferation and migration and induced tumor cell death via caspase-3 activation in the B16F10 and LLC cells, and strongly inhibited tube formation and cell migration in HUVECs. Furthermore, mouse models showed that baicalein reduced the tumor volume and greatly reduced the tumor growth rate in the early stages of tumor progression, and the baicalein-treated groups had significantly reduced expression of CD31 (endothelial cell marker) and α-SMA (mural cell marker) in the tumors, indicating that baicalein inhibits tumor angiogenesis by disrupting tumor vasculature development. Comparison of the lymph node and lung samples collected from the baicalein-treated group, and the untreated group showed that baicalein reduced metastasis of the tumor to these tissues. In summary, baicalein reduced tumor progression and metastasis, directly induced tumor cell death, and inhibited tumor angiogenesis. Our results strongly demonstrate that baicalein is a potential chemotherapeutic agent.
黄芩素是一种从黄芩根中分离出来的天然黄酮类化合物，属于草药。它具有抗癌、抗炎和神经保护作用。尽管具有这些众所周知的特性，但尚不清楚黄芩素对肿瘤进展有何影响。因此，在本研究中，我们使用 B16F10 细胞、Lewis 肺癌 (LLC) 细胞和人脐静脉内皮细胞 (HUVEC) 来研究黄芩素对细胞增殖和活力、迁移和管形成的影响。此外，还使用实验动物模型观察体内肿瘤生长速度和转移以及肿瘤血管形成。我们的结果表明，黄芩素通过 caspase-3 激活降低 B16F10 和 LLC 细胞的增殖和迁移，并强烈抑制 HUVEC 中的管形成和细胞迁移。此外，小鼠模型表明，黄芩素减少了肿瘤体积，并大大降低了肿瘤进展早期的肿瘤生长速度，黄芩素处理组的肿瘤中 CD31（内皮细胞标志物）和α-SMA（壁细胞标志物）的表达明显降低，表明黄芩素通过破坏肿瘤血管生成来抑制肿瘤血管生成。比较黄芩素处理组和未处理组收集的淋巴结和肺样本表明，黄芩素减少了肿瘤向这些组织的转移。总之，黄芩素可降低肿瘤进展和转移，直接诱导肿瘤细胞死亡，并抑制肿瘤血管生成。我们的研究结果有力地证明了黄芩素是一种有潜力的化疗药物。
