Li Xuehua, Wu Jie, Zhuang Zhanwei, Ye Yong, Zhou Shenping, Qiu Yibin, Ruan Donglin, Wang Shiyuan, Yang Jie, Wu Zhenfang, Cai Gengyuan, Zheng Enqin
College of Animal Science and National Engineering Research Center for Breeding Swine Industry, South China Agricultural University, Guangzhou 510642, China.
Guangdong Provincial Key Laboratory of Agro-Animal Genomics and Molecular Breeding, South China Agricultural University, Guangzhou 510642, China.
Animals (Basel). 2023 Feb 23;13(5):808. doi: 10.3390/ani13050808.
Internal organ weight is an essential indicator of growth status as it reflects the level of growth and development in pigs. However, the associated genetic architecture has not been well explored because phenotypes are difficult to obtain. Herein, we performed single-trait and multi-trait genome-wide association studies (GWASs) to map the genetic markers and genes associated with six internal organ weight traits (including heart weight, liver weight, spleen weight, lung weight, kidney weight, and stomach weight) in 1518 three-way crossbred commercial pigs. In summation, single-trait GWASs identified a total of 24 significant single- nucleotide polymorphisms (SNPs) and 5 promising candidate genes, namely, , , , , and as being associated with the six internal organ weight traits analyzed. Multi-trait GWAS identified four SNPs with polymorphisms localized on the , , and genes and improved the statistical efficacy of single-trait GWASs. Furthermore, our study was the first to use GWASs to identify SNPs associated with stomach weight in pigs. In conclusion, our exploration of the genetic architecture of internal organ weights helps us better understand growth traits, and the key SNPs identified could play a potential role in animal breeding programs.
内脏重量是生长状况的重要指标，因为它反映了猪的生长发育水平。然而，由于表型难以获得，相关的遗传结构尚未得到充分探索。在此，我们进行了单性状和多性状全基因组关联研究（GWAS），以定位1518头三元杂交商品猪中与六个内脏重量性状（包括心脏重量、肝脏重量、脾脏重量、肺脏重量、肾脏重量和胃重量）相关的遗传标记和基因。总之，单性状GWAS共鉴定出24个显著的单核苷酸多态性（SNP）和5个有前景的候选基因，即 、 、 、 和 ，它们与所分析的六个内脏重量性状相关。多性状GWAS鉴定出四个多态性位于 、 和 基因上的SNP，并提高了单性状GWAS的统计效力。此外，我们的研究首次使用GWAS鉴定与猪胃重量相关的SNP。总之，我们对内脏重量遗传结构的探索有助于我们更好地理解生长性状，所鉴定的关键SNP可能在动物育种计划中发挥潜在作用。
