Mantareva Vanya, Iliev Ivan, Sulikovska Inna, Durmuş Mahmut, Genova Tsanislava
Institute of Organic Chemistry with Centre of Phytochemistry, Bulgarian Academy of Sciences, Acad. G. Bonchev, Bl. 9, 1113 Sofia, Bulgaria.
Institute of Experimental Morphology, Pathology and Anthropology with Museum, Bulgarian Academy of Sciences, Bl. 25, 1113 Sofia, Bulgaria.
Gels. 2023 Jun 9;9(6):475. doi: 10.3390/gels9060475.
The conjugation of photosensitizer with collagen seems to be a very promising approach for innovative topical photodynamic therapy (PDT). The study aims to evaluate the effects of bovine collagen hydrolysate (Clg) on the properties of gallium (III) phthalocyanine (GaPc) on pigmented melanoma. The interaction of GaPc with Clg to form a conjugate (GaPc-Clg) showed a reduction of the intensive absorption Q-band (681 nm) with a blue shift of the maximum (678 nm) and a loss of shape of the UV-band (354 nm). The fluorescence of GaPc, with a strong emission peak at 694 nm was blue shifted due to the conjugation which lower intensity owing to reduce quantum yield (0.012 vs. 0.23, GaPc). The photo- and dark cytotoxicity of GaPc, Glg and GaPc-Clg on pigmented melanoma cells (SH-4) and two normal cell lines (BJ and HaCaT) showed a slight decrease of cytotoxicity for a conjugate, with low selectivity index (0.71 vs. 1.49 for GaPc). The present study suggests that the ability of collagen hydrolysate to form gels minimizes the high dark toxicity of GaPc. Collagen used for conjugation of a photosensitizer might be an essential step in advanced topical PDT.
光敏剂与胶原蛋白的结合似乎是创新型局部光动力疗法（PDT）中一种非常有前景的方法。本研究旨在评估牛胶原蛋白水解物（Clg）对色素性黑色素瘤上镓（III）酞菁（GaPc）性质的影响。GaPc与Clg相互作用形成共轭物（GaPc-Clg）后，其强吸收Q带（681 nm）强度降低，最大吸收峰蓝移至678 nm，紫外波段（354 nm）形状改变。GaPc在694 nm处有强发射峰，其荧光由于共轭作用发生蓝移，且强度降低，这是由于量子产率降低（GaPc为0.012，而之前为0.23）。GaPc、Glg和GaPc-Clg对色素性黑色素瘤细胞（SH-4）和两种正常细胞系（BJ和HaCaT）的光毒性和暗毒性表明，共轭物的细胞毒性略有降低，选择性指数较低（GaPc为0.71，之前为1.49）。本研究表明，胶原蛋白水解物形成凝胶的能力可将GaPc的高暗毒性降至最低。用于光敏剂共轭的胶原蛋白可能是先进局部光动力疗法的关键步骤。
