Department of Pharmacy, University of Naples Federico II, Via Domenico Montesano 49, 80131 Naples, Italy.
Institute of Materials Science, Kaunas University of Technology, 51423 Kaunas, Lithuania.
Int J Mol Sci. 2023 Feb 23;24(5):4449. doi: 10.3390/ijms24054449.
In this study, we fabricated three different ZnO tetrapodal nanostructures (ZnO-Ts) by a combustion process and studied their physicochemical properties by different techniques to evaluate their potentiality for label-free biosensing purposes. Then, we explored the chemical reactivity of ZnO-Ts by quantifying the available functional hydroxyl groups (-OH) on the transducer surface necessary for biosensor development. The best ZnO-T sample was chemically modified and bioconjugated with biotin as a model bioprobe by a multi-step procedure based on silanization and carbodiimide chemistry. The results demonstrated that the ZnO-Ts could be easily and efficiently biomodified, and sensing experiments based on the streptavidin target detection confirmed these structures' suitability for biosensing applications.
在这项研究中，我们通过燃烧法制备了三种不同的 ZnO 四角纳米结构（ZnO-Ts），并通过不同的技术研究了它们的物理化学性质，以评估它们在无标记生物传感方面的潜力。然后，我们通过定量测量传感器表面用于生物传感器开发的可用功能羟基（-OH）来探索 ZnO-Ts 的化学反应性。最佳的 ZnO-T 样品通过基于硅烷化和碳二亚胺化学的多步程序进行化学修饰，并与生物素进行生物共轭作为模型生物探针。结果表明，ZnO-Ts 可以很容易且有效地进行生物修饰，基于链霉亲和素靶检测的传感实验证实了这些结构适用于生物传感应用。
