Department of Applied Plant Science, Chonnam National University, Gwangju 61186, Republic of Korea.
BK21 Interdisciplinary Program in IT-Bio Convergence System, Chonnam National University, Gwangju 61186, Republic of Korea.
Int J Mol Sci. 2023 Mar 1;24(5):4762. doi: 10.3390/ijms24054762.
Cowpea ( (L.), 2 = 22) is a tropical crop grown in arid and semiarid regions that is tolerant to abiotic stresses such as heat and drought. However, in these regions, salt in the soil is generally not eluted by rainwater, leading to salt stress for a variety of plant species. This study was conducted to identify genes related to salt stress using the comparative transcriptome analysis of cowpea germplasms with contrasting salt tolerance. Using the Illumina Novaseq 6000 platform, 1.1 billion high-quality short reads, with a total length of over 98.6 billion bp, were obtained from four cowpea germplasms. Of the differentially expressed genes identified for each salt tolerance type following RNA sequencing, 27 were shown to exhibit significant expression levels. These candidate genes were subsequently narrowed down using reference-sequencing analysis, and two salt stress-related genes ( and ) with single-nucleotide polymorphism (SNP) variation were selected. Of the five SNPs identified in , one that caused significant amino acid variation was identified, while all nucleotide variations in was classified as missing in the salt-resistant germplasms. The candidate genes and their variation, identified in this study provide, useful information for the development of molecular markers for cowpea breeding programs.
菜豆（(L.)，2 = 22）是一种在干旱和半干旱地区种植的热带作物，能耐受高温和干旱等非生物胁迫。然而，在这些地区，土壤中的盐分通常不会被雨水淋洗，导致多种植物物种受到盐胁迫。本研究旨在通过对耐盐性不同的菜豆种质资源进行比较转录组分析，鉴定与盐胁迫相关的基因。使用 Illumina Novaseq 6000 平台，从四个菜豆种质中获得了 11 亿条高质量的短读序列，总长度超过 986 亿 bp。对 RNA 测序后每种耐盐类型的差异表达基因进行鉴定，发现有 27 个基因表现出显著的表达水平。随后，通过参考序列分析对这些候选基因进行了进一步的筛选，选择了两个与盐胁迫相关的基因（和），它们存在单核苷酸多态性（SNP）变异。在中鉴定出的 5 个 SNP 中，有一个导致显著的氨基酸变异，而在耐盐种质中，的所有核苷酸变异都被归类为缺失。本研究中鉴定的候选基因及其变异为菜豆育种计划提供了有用的分子标记开发信息。
