Sochacki Dariusz, Marciniak Przemysław, Ciesielska Maria, Zaród Janina
Section of Ornamental Plants, Warsaw University of Life Sciences, 166 Nowoursynowska Street, 02-787 Warsaw, Poland.
Plants (Basel). 2023 Mar 2;12(5):1134. doi: 10.3390/plants12051134.
The aim of this study was to check the effects of sugar type on the in vitro shoot multiplication of the tulip cultivar 'Heart of Warsaw' and the effects of paclobutrazol (PBZ) and 1-naphthylacetic acid (NAA) on the bulbing of previously multiplied shoots. In addition, the subsequent effects of previously used sugars on the in vitro bulb formation of this cultivar were checked. First, the optimum supplementation of Murashige and Skoog medium with plant growth regulators (PGRs) was selected for shoot multiplication. Of the six tested, the best results were obtained using a combination of 2iP 0.1 mg·L, NAA 0.1 mg·L, and mT 5.0 mg·L. The effects of different carbohydrates (sucrose, glucose, and fructose at 30 g·L and a mixture of glucose and fructose at 15 g·L each) on multiplication efficiency was then tested on this medium. The microbulb-forming experiment was carried out taking into consideration the effects of previously applied sugars, and at week 6, the agar medium was flooded with liquid medium containing NAA 2 mg·L, PBZ 1 mg·L, or medium without PGRs; in the first combination, the cultures were left on a single-phase medium, solidified with agar, as a control. After 2 months of treatment at 5 °C, the total number of microbulbs formed and the number and weights of mature microbulbs were assessed. The results obtained indicate the ability of using meta-topolin (mT) in tulip micropropagation and point to sucrose and glucose as the optimal carbohydrates for intensive shoot multiplication. The results lead to the conclusion that it is most advantageous to multiply tulip shoots on glucose medium and then to carry out cultures on a two-phase medium with PBZ, which results in a higher number of microbulbs and their faster maturation.
本研究的目的是检验糖的类型对郁金香品种‘华沙之心’离体芽增殖的影响，以及多效唑（PBZ）和1-萘乙酸（NAA）对先前增殖芽的鳞茎形成的影响。此外，还检验了先前使用的糖对该品种离体鳞茎形成的后续影响。首先，选择添加植物生长调节剂（PGR）的Murashige和Skoog培养基的最佳配方用于芽增殖。在六种测试配方中，使用2iP 0.1 mg·L、NAA 0.1 mg·L和mT 5.0 mg·L的组合获得了最佳结果。然后在该培养基上测试不同碳水化合物（30 g·L的蔗糖、葡萄糖和果糖，以及各15 g·L的葡萄糖和果糖混合物）对增殖效率的影响。考虑到先前使用的糖的影响进行了微型鳞茎形成实验，在第6周时，用含有2 mg·L NAA、1 mg·L PBZ的液体培养基或不含PGR的培养基淹没琼脂培养基；在第一种组合中，将培养物留在用琼脂固化的单相培养基上作为对照。在5℃处理2个月后，评估形成的微型鳞茎总数以及成熟微型鳞茎的数量和重量。获得的结果表明了在郁金香微繁殖中使用间- 激动素（mT）的能力，并指出蔗糖和葡萄糖是密集芽增殖的最佳碳水化合物。结果得出结论，在葡萄糖培养基上增殖郁金香芽，然后在含有PBZ的两相培养基上进行培养最为有利，这会导致产生更多的微型鳞茎且其成熟更快。
