Laboratory of Immunology, Faculty of Pharmacy, Osaka Ohtani University, Tondabayashi, Osaka, Japan.
Methods Mol Biol. 2023;2618:39-53. doi: 10.1007/978-1-0716-2938-3_3.
Dendritic cells (DCs) in peripheral tissue serve as a sentinel to invasion and maintain tolerance. They ingest and carry antigens to the draining lymph nodes and present antigens to antigen-specific T cells to initiate acquired immune responses. Thus, understanding DC migration from peripheral tissues and function is critical for understanding DCs' roles in immune homeostasis. Here, we introduced the KikGR in vivo photolabeling system, an ideal tool for monitoring precise cellular movements and related functions in vivo under physiological conditions and during various immune responses that occur in pathologic condition. Using a mouse line expressing photoconvertible fluorescent protein KikGR, we can label DCs in peripheral tissues by changing the color of KikGR from green to red after exposure to violet light and accurately track DC migration from each peripheral tissue to its respective draining lymph nodes.
树突状细胞 (DCs) 在周围组织中充当入侵的哨兵，并维持耐受。它们摄取并携带抗原到引流淋巴结，并将抗原呈递给抗原特异性 T 细胞，以启动获得性免疫反应。因此，了解 DC 从周围组织的迁移和功能对于理解 DC 在免疫稳态中的作用至关重要。在这里，我们引入了 KikGR 体内光标记系统，这是一种理想的工具，可在生理条件下以及在病理条件下发生的各种免疫反应中监测体内精确的细胞运动和相关功能。使用表达光可转换荧光蛋白 KikGR 的小鼠系，我们可以通过将 KikGR 的颜色从绿色变为红色来标记周围组织中的 DC，然后用紫光照射，从而准确跟踪 DC 从每个周围组织到其各自引流淋巴结的迁移。
