Dabrowski Bartlomiej, Zuchowska Agnieszka, Kasprzak Artur, Zukowska Grażyna Zofia, Brzozka Zbigniew
Faculty of Chemistry, Warsaw University of Technology, Poland.
Faculty of Chemistry, Warsaw University of Technology, Poland.
Chem Biol Interact. 2023 May 1;376:110444. doi: 10.1016/j.cbi.2023.110444. Epub 2023 Mar 10.
Due to its high surface area and convenient functionalization, graphene oxide has many potential applications in biomedicine, especially as a drug carrier. However, knowledge about its internalization inside mammalian cells is still limited. Graphene oxide cellular uptake is a complex phenomenon affected by factors such as the size of the particle and modifications of its surface. Moreover, nanomaterials introduced into living organisms interact with biological fluids' components. It may further alter its biological properties. All these factors must be considered when the cellular uptake of potential drug carriers is considered. In this study, the effect of graphene oxide particle sizes on internalization efficiency into normal (LL-24) and cancerous (A549) human lung cells was investigated. Moreover, one set of samples was incubated with human serum to determine how the interaction of graphene oxide with serum components affects its structure, surface, and interaction with cells. Our findings indicate that samples incubated with serum enhance cell proliferation but enter the cells with lesser efficiency than their counterparts not incubated with human serum. What is more affinity towards the cells was higher for larger particles.
由于氧化石墨烯具有高表面积和便于功能化的特点，它在生物医学领域有许多潜在应用，尤其是作为药物载体。然而，关于其在哺乳动物细胞内的内化情况的了解仍然有限。氧化石墨烯的细胞摄取是一个复杂的现象，受到颗粒大小和表面修饰等因素的影响。此外，引入生物体的纳米材料会与生物流体的成分相互作用。这可能会进一步改变其生物学特性。在考虑潜在药物载体的细胞摄取时，所有这些因素都必须加以考虑。在本研究中，研究了氧化石墨烯颗粒大小对其进入正常（LL - 24）和癌性（A549）人肺细胞的内化效率的影响。此外，将一组样品与人血清孵育，以确定氧化石墨烯与血清成分的相互作用如何影响其结构、表面以及与细胞的相互作用。我们的研究结果表明，与人血清孵育的样品可促进细胞增殖，但进入细胞的效率低于未与人血清孵育的对应样品。此外，较大颗粒对细胞的亲和力更高。
