Department of Obstetrics, Gynecology and Reproductive Sciences, University of California San Francisco, USA.
Department of Obstetrics, Gynecology and Reproductive Sciences, University of California San Francisco, USA.
J Chromatogr B Analyt Technol Biomed Life Sci. 2023 Apr 1;1220:123639. doi: 10.1016/j.jchromb.2023.123639. Epub 2023 Mar 5.
Dried blood spot (DBS) has been used as an alternative matrix in drug testing. In forensic testing it offers enhanced stability of analytes and ease of storage that requires minimal space. This is compatible with long term archiving of large numbers of samples for future investigation. We employed liquid chromatography-tandem mass spectrometry (LC-MS/MS) to quantify alprazolam, α-hydroxyalprazolam, and hydrocodone in a DBS sample that has been stored for 17 years. We achieved linear dynamic ranges (0.1-50 ng/mL) that capture wide ranges of concentration of the analytes below and above their reported reference ranges, and limits of detection (0.05 ng/mL) of 40-100X lower than the lower limit of the analyte's reference ranges. The method was validated according to FDA and CLSI guidelines and successfully confirmed and quantified alprazolam and α-hydroxyalprazolam in a forensic DBS sample.
干血斑 (DBS) 已被用作药物检测的替代基质。在法医检测中，它提供了分析物的增强稳定性和易于储存，只需要最小的空间。这与长期存档大量样本以供将来调查兼容。我们采用液相色谱-串联质谱法 (LC-MS/MS) 来定量分析干血斑样本中的阿普唑仑、α-羟基阿普唑仑和氢可酮，这些样本已经储存了 17 年。我们实现了线性动态范围 (0.1-50ng/mL)，能够捕捉到分析物浓度的广泛范围，包括低于和高于其报告的参考范围的浓度，检测限 (0.05ng/mL) 比分析物参考范围下限低 40-100 倍。该方法根据 FDA 和 CLSI 指南进行了验证，并成功地在法医 DBS 样本中确认和定量了阿普唑仑和 α-羟基阿普唑仑。
