Department of Cell and Molecular Biology, Faculty of Biological Sciences, Kharazmi University, Tehran, Iran.
Department of Cell and Molecular Biology, Faculty of Biological Sciences, Kharazmi University, Tehran, Iran.
Tissue Cell. 2021 Oct;72:101552. doi: 10.1016/j.tice.2021.101552. Epub 2021 May 8.
Ganoderma lucidum has received a lot of attention recently due to its medicinal potential activities. The aim of this designed experiment was to evaluate the beneficial effects of Ganoderma lucidum extract against lithium carbonate induced testicular toxicity and related lesions in mice testis. For this purpose, lithium carbonate at a dose of 30 mg/kg, followed by 75, 150 mg/kg Ganoderma lucidum extract orally were administered for 35 days. The results were obtained from Ganoderma lucidum extract analysis prove contained a large amount of polysaccharides, triterpenoids and poly phenols based on spectrophotometric assay. Also, DPPH assay for Ganoderma lucidum extract showed high level of radical scavenging activity. The hematoxylin & eosin cross section from lithium carbonate treated group exhibited significant alterations in seminiferous tubules. Moreover, lithium carbonate induced oxidative stress via lipid peroxidation and generate MDA (P < 0.001). In addition, lithium carbonate initiated germ cells apoptosis via increase Bax expression (p < 0.001) and reduce germ cells differentiation through down-regulation of c-Kit expression (p < 0.05). Results from CASA showed that sperm parameters like count, motility and viability significantly decreased in lithium treated group (p < 0.001). It is clear that lithium carbonate induce severe damage on male reproductive system and histopathological damages via generation oxidative stress but supplementation with Ganoderma lucidum extract exhibited prevention effects and repaired induced damages.
灵芝因具有药用潜力而受到广泛关注。本设计实验旨在评估灵芝提取物对碳酸锂诱导的睾丸毒性及相关睾丸损伤的有益作用。为此，给予 30mg/kg 的碳酸锂，随后口服给予 75、150mg/kg 的灵芝提取物，共 35 天。从灵芝提取物分析中获得的结果表明，基于分光光度法测定，灵芝提取物中含有大量的多糖、三萜类化合物和多酚。此外，灵芝提取物的 DPPH 测定显示出高水平的自由基清除活性。碳酸锂处理组的苏木精和伊红切片显示出曲细精管的显著变化。此外，碳酸锂通过脂质过氧化作用引发氧化应激，并产生 MDA（P<0.001）。此外，碳酸锂通过增加 Bax 表达（p<0.001）引发精原细胞凋亡，并通过下调 c-Kit 表达减少精原细胞分化（p<0.05）。CASA 的结果表明，锂处理组的精子参数，如计数、活力和活力明显下降（p<0.001）。很明显，碳酸锂通过产生氧化应激对雄性生殖系统和组织病理学损伤造成严重损害，但灵芝提取物的补充表现出预防作用，并修复了诱导的损伤。
