Domeni Zali Ginola, Moriel-Carretero María
Centre de Recherche en Biologie cellulaire de Montpellier (CRBM), Université de Montpellier, Centre National de la Recherche Scientifique, 34293 Montpellier CEDEX 05, France.
MicroPubl Biol. 2023 Feb 22;2023. doi: 10.17912/micropub.biology.000752. eCollection 2023.
The auxin-inducible degradation (AID) system can elicit conditional and reversible protein degradation as a tool to assess the role of essential proteins. Indeed, AID enables functional studies without the possibility of adaptation, which can occur with permanent gene deletions. The AID system relies on the addition of auxin molecules, such as indole-3-acetic acid (IAA), as a means to launch the degradation of the protein of interest. In this context, it is extremely important to control for the effect of auxin addition alone. To study the role of essential proteins in the process of selective mRNA delivery to daughter cells in , we first controlled for the effect of adding IAA to cells that cannot perform AID-mediated degradation. We found that auxin alone restricted delivery to daughter cells, as mRNA started being retained in the mother cell as soon as thirty minutes after IAA addition. Thus, our data warn about the danger of not systematically including auxin-treated cells incapable of degradation in every AID-related experiment. Furthermore, given previous data reporting the ability of auxin to inhibit the master growth regulator TORC1 in , our data suggest that TORC1 could control the selective delivery of mRNAs to daughter cells.
生长素诱导降解（AID）系统能够引发条件性和可逆性蛋白质降解，作为评估必需蛋白质作用的一种工具。事实上，AID可用于功能研究，不存在因永久基因缺失而可能出现的适应性问题。AID系统依赖于添加生长素分子，如吲哚 - 3 - 乙酸（IAA），以此启动目标蛋白质的降解。在此背景下，单独控制生长素添加的影响极为重要。为了研究必需蛋白质在[具体过程]中选择性mRNA传递至子细胞过程中的作用，我们首先对不能进行AID介导降解的细胞添加IAA的影响进行了控制。我们发现，仅生长素就限制了[具体物质]向子细胞的传递，因为在添加IAA后仅30分钟，[具体mRNA]就开始滞留在母细胞中。因此，我们的数据警示了在每个与AID相关的实验中未系统纳入不能降解的生长素处理细胞的风险。此外，鉴于先前数据报道生长素在[具体情况]中抑制主要生长调节因子TORC1的能力，我们的数据表明TORC1可能控制mRNA向子细胞的选择性传递。
