Kalpa R E, Sreejit V, Preetha R, Nagamaniammai G
Department of Food Process Engineering, School of Bioengineering, The College of Engineering and Technology, SRM Institute of Science and Technology, SRM Nagar, Kattankulathur, Chengalpattu District, 603203 Chennai, Tamil Nadu India.
J Food Sci Technol. 2023 Mar;60(3):896-905. doi: 10.1007/s13197-021-05240-z. Epub 2021 Aug 19.
Potential probiotic strains, subsp. and were microencapsulated with their appropriate prebiotics, oat bran, and oats, respectively, selected by in vitro fermentation. The microencapsulation of these probiotics were done in an alginate matrix, with and without their appropriate prebiotics. Results showed that cells microencapsulated with the prebiotics had significantly more storage stability ( < 0.05) than free cells and cells microencapsulated without the prebiotics. The probiotic cells encapsulated with their appropriate prebiotic had improved survival rates when exposed to bile as compared to free cells. The survival of microencapsulated and free cells in the simulated gastric fluid and simulated intestinal fluid was also evaluated in this study. Microencapsulated probiotics, along with an appropriate prebiotic, were found to be more stable in bile, simulated gastric fluid and simulated intestinal fluid. Interestingly, this is the first work to use prebiotic such as oats and the oat bran to prepare the synbiotic microsphere.
潜在的益生菌菌株、亚种等分别与通过体外发酵筛选出的合适益生元、燕麦麸和燕麦进行微囊化。这些益生菌的微囊化在藻酸盐基质中进行,有或没有合适的益生元。结果表明,与游离细胞和无微生元微囊化的细胞相比,用益生元微囊化的细胞具有显著更高的储存稳定性(<0.05)。与游离细胞相比,用合适益生元包封的益生菌细胞在暴露于胆汁时存活率有所提高。本研究还评估了微囊化细胞和游离细胞在模拟胃液和模拟肠液中的存活率。发现微囊化益生菌与合适的益生元一起在胆汁、模拟胃液和模拟肠液中更稳定。有趣的是,这是第一项使用燕麦和燕麦麸等益生元制备合生元微球的工作。