Reddy K Hanisha, Chandran Lekshmi, Mohan T Murali, Sudha K, Malini D L, Dominic Bonney
Department of Conservative Dentistry and Endodontics, Government Dental College and Hospital, Vijayawada, Andhra Pradesh, India.
J Conserv Dent. 2023 Jan-Feb;26(1):94-97. doi: 10.4103/jcd.jcd_417_22. Epub 2022 Dec 8.
The effect of chemical disinfection on gutta-percha (GP) has to be closely studied whether it affects surface topography and thereby causes leakage and reinfection of canals.
The purpose of the study is to assess the surface topography of GP cones after disinfecting with a novel disinfecting material, graphene oxide (GO), and compare the same with other disinfecting materials, including sodium hypochlorite (NaOCl) and silver nanoparticles (AgNps).
After determining the power (0.84) of the study, 48 GP cones were taken and categorized into four different groups based on disinfecting agents.
Forty-eight GP cones (ISO size 30 6% taper, DIADENT) were taken and are randomly selected and grouped as follows according to the disinfecting agents: Group 1 - control (untreated GP points, = 12), Group 2 - NaOCl ( = 12), Group 3 - AgNPs ( = 12), and Group 4 - GO ( = 12), respectively. After treating with the above disinfecting materials, the surface topography of samples was evaluated using atomic force microscopy. The data were analyzed statistically using one-way ANOVA and post hoc (Tukey's honestly significant difference) tests.
IBM SPSS (version 21.0) software was used. The tests performed were one-way ANOVA and post hoc. Statistically, significance was set at a ≤ 0.05.
The root means square values and surface roughness values were lesser for the GO group and AgNPs when compared with the NaOCl group, which were statistically significant.
Within the limitations of the study, this study had shown lesser surface topography deterioration of GP cones when treated with GO and AgNPs, whereas more deterioration was observed with NaOCl.
必须深入研究化学消毒对牙胶尖（GP）的影响，即其是否会影响表面形貌，进而导致根管渗漏和再感染。
本研究旨在评估用新型消毒材料氧化石墨烯（GO）消毒后牙胶尖的表面形貌，并将其与其他消毒材料（包括次氯酸钠（NaOCl）和银纳米颗粒（AgNps））进行比较。
在确定研究效能（0.84）后，选取48个牙胶尖，并根据消毒剂分为四个不同组。
选取48个牙胶尖（ISO尺寸30，锥度6%，DIADENT），随机选择并按以下消毒剂分组：第1组 - 对照组（未处理的牙胶尖，n = 12），第2组 - NaOCl（n = 12），第3组 - AgNPs（n = 12），第4组 - GO（n = 12）。用上述消毒材料处理后，使用原子力显微镜评估样品的表面形貌。数据采用单因素方差分析和事后检验（Tukey氏真实显著差异检验）进行统计学分析。
使用IBM SPSS（版本21.0）软件。进行的检验为单因素方差分析和事后检验。统计学上，显著性设定为P≤0.05。
与NaOCl组相比，GO组和AgNPs组的均方根值和表面粗糙度值较小，具有统计学意义。
在本研究的局限性范围内，本研究表明用GO和AgNPs处理时牙胶尖的表面形貌劣化较小，而用NaOCl处理时观察到更多劣化。
